Rabbit Recombinant Monoclonal IMPA1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (AB250724), expandable thumbnail](https://content.abcam.com/products/images/impa1-antibody-epr15404b-bsa-and-azide-free-ab250724--western-blot-img103677.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (AB250724)")
![Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (AB250724), expandable thumbnail](https://content.abcam.com/products/images/impa1-antibody-epr15404b-bsa-and-azide-free-ab250724--immunoprecipitation-img62759.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (AB250724)")
![Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (AB250724), expandable thumbnail](https://content.abcam.com/products/images/impa1-antibody-epr15404b-bsa-and-azide-free-ab250724--immunocytochemistry-immunofluorescence-img75376.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (AB250724)")
![Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (AB250724), expandable thumbnail](https://content.abcam.com/products/images/impa1-antibody-epr15404b-bsa-and-azide-free-ab250724--flow-cytometry-intracellular-img63430.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (AB250724)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, D-galactose 1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.
Alternative names
IMPA, IMPA1, Inositol monophosphatase 1, IMP 1, IMPase 1, D-galactose 1-phosphate phosphatase, Inositol-1(or 4)-monophosphatase 1, Lithium-sensitive myo-inositol monophosphatase A1
Recommended products
Rabbit Recombinant Monoclonal IMPA1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15404(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250724 is the carrier-free version of Anti-IMPA1 antibody [EPR15404(B)] ab184165.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The inositol monophosphatase 1 (IMPA1) also known by its alternative names Inositol-1(or 4)-monophosphatase and IMPase 1 is an enzyme that dephosphorylates inositol monophosphate to produce free inositol. IMPA1 has a molecular mass of approximately 29 kDa. Expression of IMPA1 occurs broadly in mammalian tissues with high levels in brain and kidney. The enzyme requires magnesium ions for its activity and operates efficiently under low lithium concentration.
- Biological function summary
The enzyme holds importance for phosphatidylinositol signaling and plays an important role in cellular inositol recycling. IMPA1 functions as part of a monomer or dimer but does not require complex formation with other proteins to operate effectively. Its enzyme activity maintains intracellular inositol levels which is important for cell membrane phospholipid integrity and secondary messenger systems.
- Pathways
IMPA1 is integral to the phosphoinositide signaling pathway contributing to the regeneration of inositol triphosphate (IP3) and aiding in cell signal transduction processes. It also participates in the synthesis of myo-inositol by catalyzing the final step of inositol phosphate metabolism. Within these pathways it works closely with proteins like inositol polyphosphate 1-phosphatase (INPP1) and inositol polyphosphate 5-phosphatase (INPP5).
- Associated diseases and disorders
IMPA1 has associations with bipolar disorder and schizophrenia. Lithium a common medication for bipolar disorder inhibits IMPA1 activity which affects inositol signaling. The enzyme's function and regulation impact synaptic transmission and neurochemical signaling with connections to proteins like glycogen synthase kinase 3 beta (GSK3B) in these disorders. Understanding of IMPA1's role provides insights into its potential therapeutic targets for mood disorders and other neuropsychiatric conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724), expandable thumbnail](https://content.abcam.com/products/images/impa1-antibody-epr15404b-bsa-and-azide-free-ab250724--western-blot-img103677.jpg "Western blot - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724)")
Western blot - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724)
This data was developed using Anti-IMPA1 antibody [EPR15404(B)] ab184165, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-IMPA1 antibody [EPR15404(B)] (Anti-IMPA1 antibody [EPR15404(B)] ab184165) at 1/50000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Jurkat cell lysate at 20 µg
Lane 3: PC 3 cell lysate at 20 µg
Lane 4: HCT116 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
![Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724), expandable thumbnail](https://content.abcam.com/products/images/impa1-antibody-epr15404b-bsa-and-azide-free-ab250724--immunoprecipitation-img62759.jpg "Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724)")
Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724)
This data was developed using Anti-IMPA1 antibody [EPR15404(B)] ab184165, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human testis tissue lysate (lane 1) or a PBS control (lane 2) immunoprecipitated using Anti-IMPA1 antibody [EPR15404(B)] ab184165 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.All lanes: Immunoprecipitation - Anti-IMPA1 antibody [EPR15404(B)] (Anti-IMPA1 antibody [EPR15404(B)] ab184165)
Predicted band size: 30 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724), expandable thumbnail](https://content.abcam.com/products/images/impa1-antibody-epr15404b-bsa-and-azide-free-ab250724--immunocytochemistry-immunofluorescence-img75376.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724)")
Immunocytochemistry/ Immunofluorescence - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724)
This data was developed using Anti-IMPA1 antibody [EPR15404(B)] ab184165, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HCT116 cells (4% paraformaldehyde-fixed) labeling IMPA1 with Anti-IMPA1 antibody [EPR15404(B)] ab184165 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724), expandable thumbnail](https://content.abcam.com/products/images/impa1-antibody-epr15404b-bsa-and-azide-free-ab250724--flow-cytometry-intracellular-img63430.jpg "Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724)")
Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15404(B)] - BSA and Azide free (ab250724)
This data was developed using Anti-IMPA1 antibody [EPR15404(B)] ab184165, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling IMPA1 with Anti-IMPA1 antibody [EPR15404(B)] ab184165 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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