AB191570

Anti-IMPA1 antibody [EPR15405]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal IMPA1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

IMPA, IMPA1, Inositol monophosphatase 1, IMP 1, IMPase 1, D-galactose 1-phosphate phosphatase, Inositol-1(or 4)-monophosphatase 1, Lithium-sensitive myo-inositol monophosphatase A1

4 Images

Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15405] (AB191570)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15405] (AB191570)

Intracellular flow cytometric analysis of PC3 cells labeling IMPA1 using ab191570at 1/300 dilution (red), Isotype control (green). Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Cells were fixed in 2% paraformaldehyde prior to staining.

Supplier Data

Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570)

5% NFDM/TBST blocking buffer

All lanes:

Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/100000 dilution

All lanes:

Human testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570)

5% NFDM/TBST blocking buffer

All lanes:

Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/20000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

PC3 cell lysate at 20 µg

Lane 3:

HCT 116 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570)

5% NFDM/TBST blocking buffer

All lanes:

Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/20000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Carrier free

Anti-IMPA1 antibody [EPR15405] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15405

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/100000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/300", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/100000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The inositol monophosphatase 1 (IMPA1) also known by its alternative names Inositol-1(or 4)-monophosphatase and IMPase 1 is an enzyme that dephosphorylates inositol monophosphate to produce free inositol. IMPA1 has a molecular mass of approximately 29 kDa. Expression of IMPA1 occurs broadly in mammalian tissues with high levels in brain and kidney. The enzyme requires magnesium ions for its activity and operates efficiently under low lithium concentration.

Biological function summary

The enzyme holds importance for phosphatidylinositol signaling and plays an important role in cellular inositol recycling. IMPA1 functions as part of a monomer or dimer but does not require complex formation with other proteins to operate effectively. Its enzyme activity maintains intracellular inositol levels which is important for cell membrane phospholipid integrity and secondary messenger systems.

Pathways

IMPA1 is integral to the phosphoinositide signaling pathway contributing to the regeneration of inositol triphosphate (IP3) and aiding in cell signal transduction processes. It also participates in the synthesis of myo-inositol by catalyzing the final step of inositol phosphate metabolism. Within these pathways it works closely with proteins like inositol polyphosphate 1-phosphatase (INPP1) and inositol polyphosphate 5-phosphatase (INPP5).

IMPA1 has associations with bipolar disorder and schizophrenia. Lithium a common medication for bipolar disorder inhibits IMPA1 activity which affects inositol signaling. The enzyme's function and regulation impact synaptic transmission and neurochemical signaling with connections to proteins like glycogen synthase kinase 3 beta (GSK3B) in these disorders. Understanding of IMPA1's role provides insights into its potential therapeutic targets for mood disorders and other neuropsychiatric conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, D-galactose 1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.

See full target information IMPA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 11:1498-1509 PubMed30972177

2019

Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Zhou,Ji Yang,Jinzhou Zhu,Yuming Wang,Yue Wu,Liqian Xu,Yunmei Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IMPA1 antibody [EPR15405]

Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15405] (AB191570)

Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570)

Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570)

Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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