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Rabbit Recombinant Monoclonal IMPA1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15405] (AB191570), expandable thumbnail
  • Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570), expandable thumbnail
  • Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570), expandable thumbnail
  • Western blot - Anti-IMPA1 antibody [EPR15405] (AB191570), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/10000 - 1/100000
Notes

-

Species
Human
Dilution info
1/10000 - 1/100000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/300
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, D-galactose 1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.

Alternative names

IMPA, IMPA1, Inositol monophosphatase 1, IMP 1, IMPase 1, D-galactose 1-phosphate phosphatase, Inositol-1(or 4)-monophosphatase 1, Lithium-sensitive myo-inositol monophosphatase A1

Recommended products

Rabbit Recombinant Monoclonal IMPA1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15405
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The inositol monophosphatase 1 (IMPA1) also known by its alternative names Inositol-1(or 4)-monophosphatase and IMPase 1 is an enzyme that dephosphorylates inositol monophosphate to produce free inositol. IMPA1 has a molecular mass of approximately 29 kDa. Expression of IMPA1 occurs broadly in mammalian tissues with high levels in brain and kidney. The enzyme requires magnesium ions for its activity and operates efficiently under low lithium concentration.

Biological function summary

The enzyme holds importance for phosphatidylinositol signaling and plays an important role in cellular inositol recycling. IMPA1 functions as part of a monomer or dimer but does not require complex formation with other proteins to operate effectively. Its enzyme activity maintains intracellular inositol levels which is important for cell membrane phospholipid integrity and secondary messenger systems.

Pathways

IMPA1 is integral to the phosphoinositide signaling pathway contributing to the regeneration of inositol triphosphate (IP3) and aiding in cell signal transduction processes. It also participates in the synthesis of myo-inositol by catalyzing the final step of inositol phosphate metabolism. Within these pathways it works closely with proteins like inositol polyphosphate 1-phosphatase (INPP1) and inositol polyphosphate 5-phosphatase (INPP5).

Associated diseases and disorders

IMPA1 has associations with bipolar disorder and schizophrenia. Lithium a common medication for bipolar disorder inhibits IMPA1 activity which affects inositol signaling. The enzyme's function and regulation impact synaptic transmission and neurochemical signaling with connections to proteins like glycogen synthase kinase 3 beta (GSK3B) in these disorders. Understanding of IMPA1's role provides insights into its potential therapeutic targets for mood disorders and other neuropsychiatric conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15405] (ab191570), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15405] (ab191570)

    Intracellular flow cytometric analysis of PC3 cells labeling IMPA1 using ab191570at 1/300 dilution (red), Isotype control (green). Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Cells were fixed in 2% paraformaldehyde prior to staining.

  • Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570), expandable thumbnail

    Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570)

    5% NFDM/TBST blocking buffer

    All lanes: Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/20000 dilution

    All lanes: Mouse brain tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570), expandable thumbnail

    Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570)

    5% NFDM/TBST blocking buffer

    All lanes: Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/20000 dilution

    Lane 1: Jurkat cell lysate at 20 µg

    Lane 2: PC3 cell lysate at 20 µg

    Lane 3: HCT 116 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570), expandable thumbnail

    Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570)

    5% NFDM/TBST blocking buffer

    All lanes: Western blot - Anti-IMPA1 antibody [EPR15405] (ab191570) at 1/100000 dilution

    All lanes: Human testis lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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