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AB251091

Anti-IMPA1 antibody [EPR15405] - BSA and Azide free

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Rabbit Recombinant Monoclonal IMPA1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

View Alternative Names

IMPA, IMPA1, Inositol monophosphatase 1, IMP 1, IMPase 1, D-galactose 1-phosphate phosphatase, Inositol-1(or 4)-monophosphatase 1, Lithium-sensitive myo-inositol monophosphatase A1

4 Images
Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15405] - BSA and Azide free (AB251091)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IMPA1 antibody [EPR15405] - BSA and Azide free (AB251091)

This data was developed using ab191570, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of PC3 cells labeling IMPA1 using ab191570at 1/300 dilution (red), Isotype control (green). Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Cells were fixed in 2% paraformaldehyde prior to staining.

Western blot - Anti-IMPA1 antibody [EPR15405] - BSA and Azide free (AB251091)
  • WB

Supplier Data

Western blot - Anti-IMPA1 antibody [EPR15405] - BSA and Azide free (AB251091)

This data was developed using ab191570, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IMPA1 antibody [EPR15405] (<a href='/en-us/products/primary-antibodies/impa1-antibody-epr15405-ab191570'>ab191570</a>) at 1/100000 dilution

All lanes:

Human testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Western blot - Anti-IMPA1 antibody [EPR15405] - BSA and Azide free (AB251091)
  • WB

Supplier Data

Western blot - Anti-IMPA1 antibody [EPR15405] - BSA and Azide free (AB251091)

This data was developed using ab191570, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IMPA1 antibody [EPR15405] (<a href='/en-us/products/primary-antibodies/impa1-antibody-epr15405-ab191570'>ab191570</a>) at 1/20000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

PC3 cell lysate at 20 µg

Lane 3:

HCT 116 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Western blot - Anti-IMPA1 antibody [EPR15405] - BSA and Azide free (AB251091)
  • WB

Supplier Data

Western blot - Anti-IMPA1 antibody [EPR15405] - BSA and Azide free (AB251091)

This data was developed using ab191570, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IMPA1 antibody [EPR15405] (<a href='/en-us/products/primary-antibodies/impa1-antibody-epr15405-ab191570'>ab191570</a>) at 1/20000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15405

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab251091 is the carrier-free version of ab191570.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The inositol monophosphatase 1 (IMPA1) also known by its alternative names Inositol-1(or 4)-monophosphatase and IMPase 1 is an enzyme that dephosphorylates inositol monophosphate to produce free inositol. IMPA1 has a molecular mass of approximately 29 kDa. Expression of IMPA1 occurs broadly in mammalian tissues with high levels in brain and kidney. The enzyme requires magnesium ions for its activity and operates efficiently under low lithium concentration.
Biological function summary

The enzyme holds importance for phosphatidylinositol signaling and plays an important role in cellular inositol recycling. IMPA1 functions as part of a monomer or dimer but does not require complex formation with other proteins to operate effectively. Its enzyme activity maintains intracellular inositol levels which is important for cell membrane phospholipid integrity and secondary messenger systems.

Pathways

IMPA1 is integral to the phosphoinositide signaling pathway contributing to the regeneration of inositol triphosphate (IP3) and aiding in cell signal transduction processes. It also participates in the synthesis of myo-inositol by catalyzing the final step of inositol phosphate metabolism. Within these pathways it works closely with proteins like inositol polyphosphate 1-phosphatase (INPP1) and inositol polyphosphate 5-phosphatase (INPP5).

IMPA1 has associations with bipolar disorder and schizophrenia. Lithium a common medication for bipolar disorder inhibits IMPA1 activity which affects inositol signaling. The enzyme's function and regulation impact synaptic transmission and neurochemical signaling with connections to proteins like glycogen synthase kinase 3 beta (GSK3B) in these disorders. Understanding of IMPA1's role provides insights into its potential therapeutic targets for mood disorders and other neuropsychiatric conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatase involved in the dephosphorylation of myo-inositol monophosphates to generate myo-inositol (PubMed : 17068342, PubMed : 8718889, PubMed : 9462881). Is also able to dephosphorylate scyllo-inositol-phosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-1,3-diphosphate and scyllo-inositol-1,4-diphosphate (PubMed : 17068342). Also dephosphorylates in vitro other sugar-phosphates including D-galactose-1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate and 2'-AMP (PubMed : 17068342, PubMed : 8718889, PubMed : 9462881). Responsible for the provision of inositol required for synthesis of phosphatidylinositols and polyphosphoinositides, and involved in maintaining normal brain function (PubMed : 26416544, PubMed : 8718889). Has been implicated as the pharmacological target for lithium (Li(+)) action in brain, which is used to treat bipolar affective disorder (PubMed : 17068342). Is equally active with 1D-myo-inositol 1-phosphate, 1D-myo-inositol 3-phosphate and D-galactose 1-phosphate (PubMed : 9462881).
See full target information IMPA1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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