AB69311

Anti-IMPAD1 antibody

Name: Anti-IMPAD1 antibody (ab69311) | Abcam
Brand: Abcam Limited
SKU: AB69311

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(2 Publications)

Mouse Polyclonal IMPAD1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BPNT2.

View Alternative Names

IMPA3, IMPAD1, BPNT2, Golgi-resident PAP phosphatase, gPAPP, Inositol monophosphatase domain-containing protein 1, Myo-inositol monophosphatase A3, Phosphoadenosine phosphate 3'-nucleotidase

1 Images

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Western blot - Anti-IMPAD1 antibody (AB69311)

All lanes:

Western blot - Anti-IMPAD1 antibody (ab69311) at 1/500 dilution

Lane 1:

IMPAD1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 39 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BPNT2.

Q9NX62

Specificity

This antibody is reactive against mammalian transfected lysate.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IMPAD1 also known as Inositol Monophosphatase Domain Containing 1 is an enzyme with a molecular mass of approximately 50 kDa. The enzyme is primarily located in the Golgi apparatus of cells. It catalyzes the dephosphorylation of inositol monophosphates which is a critical step in the phosphatidylinositol signaling pathway. The expression of IMPAD1 occurs in various tissues including the brain cartilage and kidney reflecting its diverse roles in different biological functions.

Biological function summary

IMPAD1 contributes to maintaining cellular inositol balance and is involved in the regulation of signaling pathways. It does not directly participate in a complex but its activity is essential for sustaining the levels of inositol phosphates within the cell. This function is particularly significant in neural and cartilage cells where it helps in the modulation of cellular responses to external stimuli.

Pathways

IMPAD1 plays a role in the phosphoinositide signaling pathway and the inositol phosphate metabolism pathway. It works alongside other phosphatases and kinases including Inositol polyphosphate-4-phosphatase type II (INPP4B) to regulate the phosphorylation state of inositol phosphates. Through these pathways it influences the synthesis of important secondary messengers like PI(345)P3 which are important for cellular signaling processes.

IMPAD1 has been associated with chondrodysplasia with joint dislocations and neurodegenerative disorders. Mutations in the IMPAD1 gene can disrupt the inositol metabolism leading to severe skeletal abnormalities and joint issues. Additionally dysfunction in its related signaling pathways particularly through interactions with proteins like INPP4B can contribute to neurodegenerative pathologies highlighting its broad impact on human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Exhibits 3'-nucleotidase activity toward adenosine 3',5'-bisphosphate (PAP), namely hydrolyzes adenosine 3',5'-bisphosphate into adenosine 5'-monophosphate (AMP) and a phosphate. May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Has no activity toward 3'-phosphoadenosine 5'-phosphosulfate (PAPS) or inositol phosphate (IP) substrates including I(1)P, I(1,4)P2, I(1,3,4)P3, I(1,4,5)P3 and I(1,3,4,5)P4.

See full target information BPNT2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111429 PubMed36170810

2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L Gibbons

Molecular therapy. Nucleic acids 29:969-978 PubMed36189081

2022

Anti-TGF-β1 aptamer enhances therapeutic effect of tyrosine kinase inhibitor, gefitinib, on non-small cell lung cancer in xenograft model.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Takahashi,Yoshifumi Hashimoto,Yoshikazu Nakamura

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IMPAD1 antibody

Western blot - Anti-IMPAD1 antibody (AB69311)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise