Anti-IMPDH2 antibody [EPR29835-594]

9 product images

• 

  Western blot - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Western blot staining using rabbit Anti-IMPDH2 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Exposure time: Lane 1-3: 48 seconds; Lane 4, 5: 180 seconds; Lane 6, 7: 59 seconds.

  All lanes: Western blot - Anti-IMPDH2 antibody [EPR29835-594] (ab323871) at 1/1000 dilution

  Lane 1: Mouse testis tissue lysate at 20 µg

  Lane 2: Mouse spleen tissue lysate at 20 µg

  Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

  Lane 4: NMuLi (mouse liver epithelial cell) whole cell lysate at 20 µg

  Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Lane 6: Rat testis tissue lysate at 20 µg

  Lane 7: Rat spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 55 kDa

• 

  Western blot - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Western blot staining using rabbit Anti-IMPDH2 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-IMPDH2 antibody [EPR29835-594] (ab323871) at 1/1000 dilution

  Lane 1: MEF (mouse embryo fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

  Lane 2: MEF transfected with siRNA specifically targeting IMPDH2 whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 55 kDa, 36 kDa

  Exposure time: 180s

• 

  Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Immunocytochemistry/ Immunofluorescence staining of MEF (mouse embryo fibroblast) using rabbit Anti-IMPDH2 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling IMPDH2 with ab323871 at 1/50 (9.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing mainly cytoplasmic staining in MEF cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Immunocytochemistry/ Immunofluorescence staining of PC-12 (rat adrenal gland pheochromocytoma cell) using rabbit Anti-IMPDH2 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling IMPDH2 with ab323871 at 1/50 (9.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing mainly cytoplasmic staining in PC-12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen using rabbit Anti-IMPDH2 antibody

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling IMPDH2 with ab323871 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse spleen (PMID: 30555474).
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse kidney using rabbit Anti-IMPDH2 antibody

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling IMPDH2 with ab323871 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse kidney.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis using rabbit Anti-IMPDH2 antibody

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling IMPDH2 with ab323871 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse testis.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse breast cancer using rabbit Anti-IMPDH2 antibody

  Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling IMPDH2 with ab323871 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse breast cancer.
  
  The primary antibody was incubated for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Dot Blot - Anti-IMPDH2 antibody [EPR29835-594] (ab323871)

  IMPDH2 Dot Blot staining using rabbit Anti-IMPDH2 antibody

  Dot blot analysis of IMPDH2 using ab323871 at 1:1000 (0.488 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

  Lane 1: Mouse IMPDH2 recombinant protein
  
  Lane 2: C-Myc/DDK Mouse IMPDH1 recombinant protein

  Exposure time: 3 minutes.

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  This antibody doesn't cross-react with IMPDH1.
  
  Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] staining at 1/1000 dilution.

  All lanes: Dot Blot - Anti-IMPDH2 antibody [EPR29835-594] (ab323871) at 1/1000 dilution

  Lane 1: Mouse IMPDH2 recombinant protein

  Lane 2: C-Myc/DDK Mouse IMPDH1 recombinant protein

  Secondary

  All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Exposure time: 180s