Rabbit Recombinant Monoclonal IMPDH2 antibody. Carrier free. Suitable for Dot, ICC/IF, IHC-P, WB and reacts with Recombinant full length protein - Mouse, Mouse, Rat samples.
Images
![Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--western-blot-img446161.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877)")
![Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--western-blot-img446160.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877)")
![Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunocytochemistry-immunofluorescence-img446184.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877)")
![Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunocytochemistry-immunofluorescence-img446185.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446209.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (AB323877)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|
| Mouse | Expected | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected | Tested |
| Recombinant full length protein - Mouse | Tested | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Target data
Function
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
Additional Targets
Inosine-5'-monophosphate dehydrogenase 2
Alternative names
Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, IMPDH-II
Rabbit Recombinant Monoclonal IMPDH2 antibody. Carrier free. Suitable for Dot, ICC/IF, IHC-P, WB and reacts with Recombinant full length protein - Mouse, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29835-594
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab323877 is the carrier free version of Anti-IMPDH2 antibody [EPR29835-594] ab323871.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--western-blot-img446161.jpg "Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Western blot staining using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-3: 48 seconds; Lane 4, 5: 180 seconds; Lane 6, 7: 59 seconds.
All lanes: Western blot - Anti-IMPDH2 antibody [EPR29835-594] (Anti-IMPDH2 antibody [EPR29835-594] ab323871) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Lane 4: NMuLi (mouse liver epithelial cell) whole cell lysate at 20 µg
Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 6: Rat testis tissue lysate at 20 µg
Lane 7: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
![Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--western-blot-img446160.jpg "Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Western blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Western blot staining using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-IMPDH2 antibody [EPR29835-594] (Anti-IMPDH2 antibody [EPR29835-594] ab323871) at 1/1000 dilution
Lane 1: MEF (mouse embryo fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: MEF transfected with siRNA specifically targeting IMPDH2 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa, 36 kDa
Exposure time: 180s
![Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunocytochemistry-immunofluorescence-img446184.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Immunocytochemistry/ Immunofluorescence staining of MEF (mouse embryo fibroblast) using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling IMPDH2 with Anti-IMPDH2 antibody [EPR29835-594] ab323871 at 1/50 (9.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing mainly cytoplasmic staining in MEF cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunocytochemistry-immunofluorescence-img446185.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Immunocytochemistry/ Immunofluorescence staining of PC-12 (rat adrenal gland pheochromocytoma cell) using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling IMPDH2 with Anti-IMPDH2 antibody [EPR29835-594] ab323871 at 1/50 (9.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing mainly cytoplasmic staining in PC-12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446209.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling IMPDH2 with Anti-IMPDH2 antibody [EPR29835-594] ab323871 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen (PMID: 30555474).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446210.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse kidney using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling IMPDH2 with Anti-IMPDH2 antibody [EPR29835-594] ab323871 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse kidney.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446211.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling IMPDH2 with Anti-IMPDH2 antibody [EPR29835-594] ab323871 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse testis.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446212.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse breast cancer using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling IMPDH2 with Anti-IMPDH2 antibody [EPR29835-594] ab323871 at 1/2000 (0.244 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse breast cancer.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Dot Blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--dot-blot-img446244.jpg "Dot Blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
Dot Blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)
IMPDH2 Dot Blot staining using rabbit Anti-IMPDH2 antibody
This data was developed using Anti-IMPDH2 antibody [EPR29835-594] ab323871, the same antibody clone in a different buffer formulation.
Dot blot analysis of IMPDH2 using Anti-IMPDH2 antibody [EPR29835-594] ab323871 at 1:1000 (0.488 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: Mouse IMPDH2 recombinant protein
Lane2: C-Myc/DDK Mouse IMPDH1 recombinant proteinExposure time: 3 minutes.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody doesn't cross-react with IMPDH1.
Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] staining at 1/1000 dilution.All lanes: Dot Blot - Anti-IMPDH2 antibody [EPR29835-594] (Anti-IMPDH2 antibody [EPR29835-594] ab323871) at 1/1000 dilution
Lane 1: Mouse IMPDH2 recombinant protein
Lane 2: C-Myc/DDK Mouse IMPDH1 recombinant protein
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446210.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446211.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446212.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
![Dot Blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877), expandable thumbnail](https://content.abcam.com/products/images/impdh2-antibody-epr29835-594-bsa-and-azide-free-ab323877--dot-blot-img446244.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-IMPDH2 antibody [EPR29835-594] - BSA and Azide free (ab323877)")
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