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Rabbit Recombinant Monoclonal IMPDH2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 15 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (AB131158), expandable thumbnail
  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (AB131158), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (AB131158), expandable thumbnail
  • OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (AB131158), expandable thumbnail
  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (AB131158), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (PubMed:7763314, PubMed:7903306). Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism (PubMed:14766016). It may also have a role in the development of malignancy and the growth progression of some tumors.

Alternative names

IMPD2, IMPDH2, Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, Inosine-5'-monophosphate dehydrogenase type II, IMP dehydrogenase II, IMPDH-II

Recommended products

Rabbit Recombinant Monoclonal IMPDH2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 15 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8364(B)
Purification technique
Affinity purification Protein A
Specificity

The immunogen used to raise this antibody shares 79% homology with Human IMPDH1.

This antibody shows very faint band when testing IMPDH1 recombinant protein in WB test.

Dissociation constant
2.2 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IMPDH2 also known as inosine monophosphate dehydrogenase 2 plays an important role in the de novo synthesis of guanine nucleotides. This enzyme with a molecular mass of approximately 56 kDa catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) an important step in the nucleotide biosynthesis. IMPDH2 is expressed in various tissues across the body with high expression in proliferating cells due to increased nucleotide requirements.

Biological function summary

IMPDH2 functions as a homotetramer forming part of a larger complex that regulates nucleotide levels. This regulation is critical for cell growth differentiation and proliferation. IMPDH2 activity drives the synthesis of guanine nucleotides which are essential for DNA and RNA synthesis influencing cellular communication and energy transfer.

Pathways

The target IMPDH2 has a significant role in the purine nucleotide biosynthesis pathway aligning with other enzymes like GMP synthetase. This pathway is critical in maintaining cellular nucleotide pools. IMPDH2’s activity closely interacts with guanosine monophosphate reductase within the same pathway ensuring the balance between different types of nucleotides necessary for various cellular processes.

Associated diseases and disorders

Aberrations in IMPDH2 activity link to immunological diseases and cancer. Overexpression or upregulation of this enzyme contributes to the rapid proliferation seen in cancerous cells by maintaining high guanine nucleotide levels. Additionally IMPDH2 serves as a target for immunosuppressive drugs used in autoimmune conditions highlighting its relationship with T- and B-cell proliferation. This positions IMPDH2 in therapeutic strategies alongside its pathway partners to modulate disease outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158), expandable thumbnail

    Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    All lanes: Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution

    Lane 1: K562 cell lysate

    Lane 2: HepG2 cell lysate

    Lane 3: HeLa cell lysate

    Lane 4: NCI-H460 cell lysate

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158), expandable thumbnail

    OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158), expandable thumbnail

    Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times:

    Lane 1&2: 5 seconds

    Lane 3: 180 seconds.

    This antibody shows very faint band when testing IMPDH1 recombinant protein in WB test

    All lanes: Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution

    Lanes 1 and 3: C-Myc/DDK tagged full length IMPDH1 Human Recombinant Protein

    Lane 2: C-Myc/DDK tagged full length IMPDH2 Human Recombinant Protein

    Predicted band size: 56 kDa

    Observed band size: 75 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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