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Rabbit Recombinant Monoclonal IMPDH2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (AB248323), expandable thumbnail
  • Western blot - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (AB248323), expandable thumbnail
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (AB248323), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (AB248323), expandable thumbnail
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (AB248323), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Predicted
Predicted
Not recommended
Predicted
Predicted
Rat
Predicted
Predicted
Not recommended
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

Target data

Function

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (PubMed:7763314, PubMed:7903306). Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism (PubMed:14766016). It may also have a role in the development of malignancy and the growth progression of some tumors.

Alternative names

IMPD2, IMPDH2, Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, Inosine-5'-monophosphate dehydrogenase type II, IMP dehydrogenase II, IMPDH-II

Recommended products

Rabbit Recombinant Monoclonal IMPDH2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8365(B)
Purification technique
Affinity purification Protein A
Specificity

The immunogen used to raise this antibody shares 86% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally.

Dissociation constant
2.24 x 10-9 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248323 is the carrier-free version of Anti-IMPDH2 antibody [EPR8365(B)] ab129165.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IMPDH2 also known as inosine monophosphate dehydrogenase 2 plays an important role in the de novo synthesis of guanine nucleotides. This enzyme with a molecular mass of approximately 56 kDa catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) an important step in the nucleotide biosynthesis. IMPDH2 is expressed in various tissues across the body with high expression in proliferating cells due to increased nucleotide requirements.

Biological function summary

IMPDH2 functions as a homotetramer forming part of a larger complex that regulates nucleotide levels. This regulation is critical for cell growth differentiation and proliferation. IMPDH2 activity drives the synthesis of guanine nucleotides which are essential for DNA and RNA synthesis influencing cellular communication and energy transfer.

Pathways

The target IMPDH2 has a significant role in the purine nucleotide biosynthesis pathway aligning with other enzymes like GMP synthetase. This pathway is critical in maintaining cellular nucleotide pools. IMPDH2’s activity closely interacts with guanosine monophosphate reductase within the same pathway ensuring the balance between different types of nucleotides necessary for various cellular processes.

Associated diseases and disorders

Aberrations in IMPDH2 activity link to immunological diseases and cancer. Overexpression or upregulation of this enzyme contributes to the rapid proliferation seen in cancerous cells by maintaining high guanine nucleotide levels. Additionally IMPDH2 serves as a target for immunosuppressive drugs used in autoimmune conditions highlighting its relationship with T- and B-cell proliferation. This positions IMPDH2 in therapeutic strategies alongside its pathway partners to modulate disease outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323)

    This data was developed using Anti-IMPDH2 antibody [EPR8365(B)] ab129165, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labelling IMPDH2 (Green) with Anti-IMPDH2 antibody [EPR8365(B)] ab129165 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG H&L, was used as the secondary antibody at a 1/1000. Tubulin (Red) was stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, an Alexa Fluor® 594-conjugated anti-alpha tubulin antibody, at a 1/200 dilution. DAPI (Blue) was used as the nuclear counterstain. Negative control using only secondary antibody.

    Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323), expandable thumbnail

    Western blot - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323)

    This data was developed using Anti-IMPDH2 antibody [EPR8365(B)] ab129165, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-IMPDH2 antibody [EPR8365(B)] (Anti-IMPDH2 antibody [EPR8365(B)] ab129165) at 1/1000 dilution

    Lane 1: DDDK tagged human full length IMPDH1 recombinant protein at 10 µg

    Lane 2: DDDK tagged human full length IMPDH2 recombinant protein at 10 µg

    Secondary

    All lanes: NFDM/TBST at 1/20000 dilution

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

    Exposure time: 140s

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323), expandable thumbnail

    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323)

    This data was developed using Anti-IMPDH2 antibody [EPR8365(B)] ab129165, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling IMPDH2 with Anti-IMPDH2 antibody [EPR8365(B)] ab129165 at 1/4000 for 30 mins at room temperature. A Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody.

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Negative control using only secondary antibody. Counterstained with hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Flow Cytometry (Intracellular) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323)

    This data was developed using Anti-IMPDH2 antibody [EPR8365(B)] ab129165, the same antibody clone in a different buffer formulation.

    Intracellular Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling IMPDH2 with Anti-IMPDH2 antibody [EPR8365(B)] ab129165 at 1/20 dilution (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. Rabbit monoclonal - Alexa Fluor® 488 (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323), expandable thumbnail

    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323)

    This data was developed using Anti-IMPDH2 antibody [EPR8365(B)] ab129165, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling IMPDH2 with Anti-IMPDH2 antibody [EPR8365(B)] ab129165 at 1/4000 for 30 mins at room temperature. A Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody.

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Negative control using only secondary antibody. Counterstained with hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323), expandable thumbnail

    Western blot - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323)

    This data was developed using Anti-IMPDH2 antibody [EPR8365(B)] ab129165, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lanes 1 - 6: Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (Anti-Met (c-Met) antibody [EP1454Y] - N-terminal ab51067) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-IMPDH2 antibody [EPR8365(B)] (Anti-IMPDH2 antibody [EPR8365(B)] ab129165) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 1: DDDK tagged human full length IMPDH1 recombinant protein at 10 µg

    Lane 2: MET knockout A549 cell lysate at 20 µg

    Lane 2: DDDK tagged human full length IMPDH2 recombinant protein at 10 µg

    Lane 3: Wild-type HeLa ab255929 cell lysate at 20 µg

    Lane 4: MET (Met (c-Met)) knockout HeLa Human MET (c-Met) knockout HeLa cell lysate ab256991 cell lysate at 20 µg

    Lane 5: HT-29 cell lysate at 20 µg

    Lane 6: T-47D cell lysate at 20 µg

    Secondary

    All lanes: NFDM/TBST at 1/20000 dilution

    Observed band size: 40-50 kDa

  • Western blot - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323), expandable thumbnail

    Western blot - Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free (ab248323)

    This data was developed using Anti-IMPDH2 antibody [EPR8365(B)] ab129165, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-IMPDH2 antibody [EPR8365(B)] (Anti-IMPDH2 antibody [EPR8365(B)] ab129165) at 1/10000 dilution

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 3 - 4: Rat spleen lysate at 20 µg

    Lane 5: Mouse spleen lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

    Exposure time: 60s

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