AB137112

Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal IMPDH1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

IMPD1, IMPDH1, Inosine-5'-monophosphate dehydrogenase 1, IMP dehydrogenase 1, IMPD 1, IMPDH 1, IMPDH-I, IMPD2, IMPDH2, Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, Inosine-5'-monophosphate dehydrogenase type II, IMP dehydrogenase II, IMPDH-II

3 Images

Flow Cytometry (Intracellular) - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (AB137112)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (AB137112)

Intracellular flow cytometric analysis of permeabilized K562 cells labelling IMPDH1 with ab137112 (red) at 1/500 dilution or a rabbit IgG (negative) (green).

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Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (AB137112)

All lanes:

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (ab137112) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP-labeled goat anti-rabbit at 1/2000 dilution

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Supplier Data

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (AB137112)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (ab137112) at 1/1000 dilution

Lane 1:

C-Myc/DDK tagged full length IMPDH1 Human Recombinant Protein

Lane 2:

C-Myc/DDK tagged full length IMPDH2 Human Recombinant Protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 75 kDa

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Exposure time: 20s

Carrier free

Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9679(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The proteins IMPDH2 and IMPDH1 also known as inosine-5'-monophosphate dehydrogenase 2 and 1 are enzymes involved in purine metabolism. IMPDH1 has a mass of approximately 55 kDa while IMPDH2 measures around 56 kDa. Both proteins catalyze the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP) an essential step in the de novo synthesis of guanine nucleotides. They are expressed in various tissues with highest levels found in blood and immune system cells reflecting their important role in cell proliferation.

Biological function summary

These enzymes perform critical functions by facilitating the synthesis of guanine nucleotides which are building blocks of DNA and RNA. IMPDH1 and IMPDH2 do not function within large multimeric complexes but exhibit regulatory roles over cellular proliferation and differentiation. The balance between these enzymes impacts the overall guanine nucleotide pool affecting cellular processes such as growth and division.

Pathways

These enzymes link to nucleotide biosynthesis pathways particularly the guanine nucleotide synthesis pathway. This pathway integrates with the purine metabolism network. IMPDH proteins interact with key enzymes like GMP synthase which directly follows IMPDHs in nucleotide synthesis. Their activity regulates cell proliferative capacity making them integral to processes like DNA replication and repair.

The proteins play significant roles in conditions like cancer and retinitis pigmentosa. IMPDH2 is often overexpressed in rapidly proliferating cancer cells linking it to tumor growth and progression. Meanwhile mutations in IMPDH1 have associations with retinitis pigmentosa a genetic disorder causing retinal degeneration. These enzymes’ dysfunction relates to proteins they interact with in these diseases such as DNA repair proteins in cancer and photoreceptor proteins in retinal disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.

See full target information IMPDH1

Additional targets

IMPDH2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5209 PubMed40473625

2025

Lysosomal TMEM165 controls cellular ion homeostasis and survival by mediating lysosomal Ca import and H efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Chen,Bin Liu,Dawid Jaślan,Lucija Kucej,Veronika Kudrina,Belinda Warnke,Yvonne E Klingl,Arnas Petrauskas,Sandra Prat Castro,Kenji Maeda,Christian Grimm,Marja Jäättelä

Redox biology 84:103649 PubMed40446642

2025

PRPS activity tunes redox homeostasis in Myc-driven lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Austin C MacMillan,Bibek Karki,Juechen Yang,Karmela R Gertz,Samantha Zumwalde,Jay G Patel,Maria F Czyzyk-Krzeska,Jarek Meller,John T Cunningham

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)]

Flow Cytometry (Intracellular) - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (AB137112)

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (AB137112)

Western blot - Anti-IMPDH2 + IMPDH1 antibody [EPR9679(B)] (AB137112)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise