Rabbit Polyclonal IMPG1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IMPG1 aa 200-450.
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- Acta biomaterialia 74:207-2212018Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMajed Felemban et. al.PubMed 29777959
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IMPG1 aa 200-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q17R60
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Chondroitin sulfate-, heparin- and hyaluronan-binding protein (By similarity). May serve to form a basic macromolecular scaffold comprising the insoluble interphotoreceptor matrix (PubMed:9813076).
Alternative names
IPM150, SPACR, IMPG1, Interphotoreceptor matrix proteoglycan 1, Interphotoreceptor matrix proteoglycan of 150 kDa, Sialoprotein associated with cones and rods, IPM-150
Recommended products
Rabbit Polyclonal IMPG1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IMPG1 aa 200-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IMPG1 aa 200-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q17R60
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Reconstitute in 200 µL of water
- Concentration
- Purification notes
ab113493 is purified by a peptide affinity column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IMPG1 also known as Interphotoreceptor Matrix Proteoglycan 1 or SPACR (Sialoprotein Associated with Cones and Rods) is a protein with a mass of about 133 kDa. It plays a mechanical role in the structure and function of the interphotoreceptor matrix (IPM) in the eye. IMPG1 locates between the photoreceptor cells and the retinal pigment epithelium and its expression is highly specific to retinal tissues. This protein contributes to maintaining the extracellular environment important for ocular photoreceptors.
- Biological function summary
IMPG1 supports the stability and integrity of the IPM serving as a structural component and interacting with various molecules within the matrix. It contributes to the arrangement of photoreceptor outer segments facilitating their proper function. IMPG1 forms a complex with other glycoproteins in the IPM providing a scaffold for adhesion molecules and playing a significant role in visual cycles. Its interactions with retinal pigment epithelial cells influence photoreceptor health and homeostasis.
- Pathways
IMPG1 participates prominently in the visual cycle and phototransduction pathway. In these pathways the protein ensures effective communication and nutrient exchange between photoreceptors and retinal pigment epithelial cells. Notably IMPG1 interacts with peripherin-2 a protein essential for disc morphogenesis in photoreceptors. This relationship enhances the structural cohesion needed for efficient phototransduction and maintenance of vision.
- Associated diseases and disorders
IMPG1 associates strongly with retinitis pigmentosa and macular degeneration. These conditions often involve mutations or malfunctions in this protein leading to progressive vision loss. IMPG1 relationships with proteins like peripherin-2 are critical in understanding such diseases as disruptions can impair the protective and structural roles of the interphotoreceptor matrix. Studying these connections aids in developing potential therapeutic strategies for degenerative retinal disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-IMPG1 antibody (ab113493)
All lanes: Western blot - Anti-IMPG1 antibody (ab113493) at 1/500 dilution
Lane 1: Human fetal liver lysate
Lane 2: Human fetal muscle lysate
Lane 3: Human fetal kidney lysate
Lane 4: HepG2 cell lysate
Lane 5: 97 H cell lysate
Lane 6: LO2 cell lysate
Predicted band size: 89 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPG1 antibody (ab113493)
ab113493 at 1/100 dilution staining IMPG1 in Formalin-fixed, Paraffin-embedded Human fetal liver tissue by Immunohistochemistry.
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