AB101374

Anti-Importin 13 antibody

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(1 Publication)

Rabbit Polyclonal Importin 13 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IPO13 aa 100-300.

View Alternative Names

KIAA0724, RANBP13, IPO13, Importin-13, Imp13, Karyopherin-13, Ran-binding protein 13, Kap13, RanBP13

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 13 antibody (AB101374)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 13 antibody (AB101374)

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab101374 at 1/500 dilution.

Unknown

Western blot - Anti-Importin 13 antibody (AB101374)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Importin 13 antibody (ab101374) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 108 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IPO13 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

O94829

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Importin 13 also known as IPO13 is a karyopherin with a molecular mass of approximately 100 kDa. This protein shuttles cargo between the cytoplasm and nucleus facilitating the nuclear import of various proteins and some RNAs. It expresses in many tissues with higher levels observed in brain testes and heart. Importin 13's transport function plays a significant role in regulating nuclear-cytoplasmic trafficking which is critical for cellular function and communication.

Biological function summary

The role of Importin 13 extends beyond simple transportation. It interacts with other proteins to regulate transcription and is sometimes part of a larger protein complex. Importin 13 helps in the import of transcription factors and other regulatory molecules into the nucleus influencing gene expression that affects cell differentiation and development. Its activity contributes to maintaining proper cellular states and transitions impacting processes such as neurogenesis and spermatogenesis.

Pathways

Importin 13 is intimately involved in the MAPK/ERK signaling pathway which determines cell growth and differentiation. It closely interacts with proteins like ERK2 facilitating their correct localization and function within the cell. These interactions ensure the precise regulation and execution of signaling events within the pathway enabling cells to respond correctly to extracellular signals. Additionally Importin 13 participates in the Wnt/β-catenin pathway further affecting cellular growth and development.

Importin 13's dysfunction connects to neurological disorders and cancer. Abnormal expression or mutations can contribute to developmental brain disorders due to its impact on neurogenesis. It has associations with cancer especially through pathways involving ERK2 and β-catenin where improper localization and activation might lead to uncontrolled cell growth. Studying Importin 13 offers insights into these conditions prompting potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13.

See full target information IPO13

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 6:7390 PubMed26077467

2015

Programmed cell death 5 mediates HDAC3 decay to promote genotoxic stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Kyoung Choi,Youngsok Choi,Eun Sung Park,Soo-Yeon Park,Seung-Hyun Lee,Jaesung Seo,Mi-Hyeon Jeong,Jae-Wook Jeong,Jae-Ho Jeong,Peter C W Lee,Kyung-Chul Choi,Ho-Geun Yoon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Importin 13 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 13 antibody (AB101374)

Western blot - Anti-Importin 13 antibody (AB101374)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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