Rabbit Polyclonal Importin 13 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Transfected cell lysate samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IPO13 aa 900 to C-terminus.
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Publications
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- Journal of cell science 134:2021On the asymmetric partitioning of nucleocytoplasmic transport - recent insights and open questions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoanna Kalita,Larisa E Kapinos,Roderick Y H LimPubMed 33912945
- The Journal of biological chemistry 290:18662-702015Forkhead Box M1 Is Essential for Nuclear Localization of Glioma-associated Oncogene Homolog 1 in Glioblastoma Multiforme Cells by Promoting Importin-7 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianfei Xue et. al.PubMed 26085085
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human IPO13 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O94829
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Transfected cell lysate | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-10 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13.
Alternative names
Importin-13, Imp13, Karyopherin-13, Ran-binding protein 13, Kap13, RanBP13, RANBP13, KIAA0724, IPO13
Recommended products
Rabbit Polyclonal Importin 13 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Transfected cell lysate samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IPO13 aa 900 to C-terminus.
Alternative names
Importin-13, Imp13, Karyopherin-13, Ran-binding protein 13, Kap13, RanBP13, RANBP13, KIAA0724, IPO13
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human IPO13 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O94829
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Importin 13 also known as IPO13 is a karyopherin with a molecular mass of approximately 100 kDa. This protein shuttles cargo between the cytoplasm and nucleus facilitating the nuclear import of various proteins and some RNAs. It expresses in many tissues with higher levels observed in brain testes and heart. Importin 13's transport function plays a significant role in regulating nuclear-cytoplasmic trafficking which is critical for cellular function and communication.
- Biological function summary
The role of Importin 13 extends beyond simple transportation. It interacts with other proteins to regulate transcription and is sometimes part of a larger protein complex. Importin 13 helps in the import of transcription factors and other regulatory molecules into the nucleus influencing gene expression that affects cell differentiation and development. Its activity contributes to maintaining proper cellular states and transitions impacting processes such as neurogenesis and spermatogenesis.
- Pathways
Importin 13 is intimately involved in the MAPK/ERK signaling pathway which determines cell growth and differentiation. It closely interacts with proteins like ERK2 facilitating their correct localization and function within the cell. These interactions ensure the precise regulation and execution of signaling events within the pathway enabling cells to respond correctly to extracellular signals. Additionally Importin 13 participates in the Wnt/β-catenin pathway further affecting cellular growth and development.
- Associated diseases and disorders
Importin 13's dysfunction connects to neurological disorders and cancer. Abnormal expression or mutations can contribute to developmental brain disorders due to its impact on neurogenesis. It has associations with cancer especially through pathways involving ERK2 and β-catenin where improper localization and activation might lead to uncontrolled cell growth. Studying Importin 13 offers insights into these conditions prompting potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Importin 13 antibody (ab95993)
All lanes: Western blot - Anti-Importin 13 antibody (ab95993)
Lane 1: Non-transfected lysate
Lane 2: IPO13 transfected lysate
Predicted band size: 108 kDa
Immunoprecipitation - Anti-Importin 13 antibody (ab95993)
Importin 13 was immunoprecipitated from 1 mg of HeLa whole cell lysate using 10 μg of ab95993. The precipitated Importin 13 was detected with ab95993 at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-Importin 13 antibody (ab95993)
Predicted band size: 108 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Importin 13 antibody (ab95993)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab95993 at 1/200 dilution. Lower image is merged with DNA probe.
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