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Rabbit Recombinant Monoclonal Importin 9/RANBP9 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710), expandable thumbnail
  • Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710), expandable thumbnail
  • OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Expected
Not recommended
Not recommended
Tested
Rat
Expected
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/500 - 1/1000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Nuclear transport receptor that mediates nuclear import of proteins, such as histones, proteasome and actin (PubMed:11823430, PubMed:30855230, PubMed:34711951). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11823430). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11823430). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed:11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11823430). Mediates the import of pre-assembled proteasomes into the nucleus; AKIRIN2 acts as a molecular bridge between IPO9 and the proteasome complex (PubMed:11823430, PubMed:34711951). Mediates the nuclear import of histones H2A, H2B, H4 and H4 (PubMed:11823430, PubMed:30855230). In addition to nuclear import, also acts as a chaperone for histones by preventing inappropriate non-nucleosomal interactions (PubMed:30855230). Mediates the nuclear import of actin (By similarity).

Alternative names

IMP9, KIAA1192, RANBP9, HSPC273, IPO9, Importin-9, Imp9, Ran-binding protein 9, RanBP9

Recommended products

Rabbit Recombinant Monoclonal Importin 9/RANBP9 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1352
Purification technique
Affinity purification Protein A
Dissociation constant
2.29 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Importin 9 also known as RANBP9 or IPO9 is a nuclear transport receptor with a molecular mass of approximately 107 kDa. This protein facilitates the transport of molecules into the nucleus a process essential for cellular function. Importin 9 is expressed in various tissues and plays a significant role in shuttling proteins and some RNA species across the nuclear envelope. As a member of the importin family it interacts with nuclear localization signals on target proteins steering them through nuclear pore complexes.

Biological function summary

Importin 9 participates in intracellular transport by binding cargo proteins in the cytoplasm and transporting them into the nucleus. It operates as part of the larger nuclear transport complex which relies on the Ran GTPase system for direction and timing. Importin 9's role is pivotal in regulating gene expression and cell cycle activities as transported molecules often influence transcriptional activity within the nucleus. This regulation assists in maintaining normal cellular functions and adaptations to stress signals.

Pathways

Importin 9 is integral to the nucleocytoplasmic transport pathway essential for several cellular processes including the regulation of signal transduction and chromatin remodeling. Ran GTPase facilitates the release of cargo once Importin 9 reaches the nucleus while the protein Importin β plays a cooperative role in many pathways. Together they ensure the correct localization of proteins necessary for DNA replication and repair highlighting Importin 9's role in DNA-related processes.

Associated diseases and disorders

Dysfunction of Importin 9 links to neurological conditions and cancer. Abnormal regulation of nuclear transport may contribute to the pathogenesis of neurodegenerative diseases where Importin 9 collaborates with Tau protein in neuronal cells. In oncology altered Importin 9 activity has connections to tumorigenesis often involving the c-Myc protein where misregulated nuclear transport leads to uncontrolled cell proliferation and cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710), expandable thumbnail

    Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

    Lanes 1-3: Merged signal (red and green). Green - ab124710. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human IPO9 (Importin 9/RANBP9) knockout HeLa cell line ab265352 (knockout cell lysate Human IPO9 (Importin 9/RANBP9) knockout HeLa cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: IPO9 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human IPO9 (Importin 9/RANBP9) knockout HeLa cell line (Human IPO9 (Importin 9/RANBP9) knockout HeLa cell line ab265352)

    Lane 3: SK-BR-3 cell lysate at 20 µg

    Predicted band size: 116 kDa

  • Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710), expandable thumbnail

    Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

    All lanes: Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: SKBR-3 cell lysate at 10 µg

    Lane 3: C6 cell lysate at 10 µg

    Lane 4: NIH/3T3 cell lysate at 10 µg

    Predicted band size: 116 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

    ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710), expandable thumbnail

    OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

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