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AB124710

Anti-Importin 9/RANBP9 antibody [EPR1352]

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(2 Publications)

Rabbit Recombinant Monoclonal Importin 9/RANBP9 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

IMP9, KIAA1192, RANBP9, HSPC273, IPO9, Importin-9, Imp9, Ran-binding protein 9, RanBP9

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710)

ab124710, at 1/500 to 1/1000, staining Impotin 9/RANBP9 in Human brain tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710)
  • WB

Unknown

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710)

All lanes:

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SKBR-3 cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Predicted band size: 116 kDa

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Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710)
  • WB

Supplier Data

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710)

Lanes 1-3 : Merged signal (red and green). Green - ab124710. Red - loading control, ab8245 observed at 36 kDa.

ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (ab124710) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

IPO9 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human IPO9 (Importin 9/RANBP9) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ipo9-importin-9-ranbp9-knockout-hela-cell-line-ab265352'>ab265352</a>)

Lane 3:

SK-BR-3 cell lysate at 20 µg

Predicted band size: 116 kDa

false

OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Importin 9/RANBP9 antibody [EPR1352] (AB124710)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1352

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Importin 9 also known as RANBP9 or IPO9 is a nuclear transport receptor with a molecular mass of approximately 107 kDa. This protein facilitates the transport of molecules into the nucleus a process essential for cellular function. Importin 9 is expressed in various tissues and plays a significant role in shuttling proteins and some RNA species across the nuclear envelope. As a member of the importin family it interacts with nuclear localization signals on target proteins steering them through nuclear pore complexes.
Biological function summary

Importin 9 participates in intracellular transport by binding cargo proteins in the cytoplasm and transporting them into the nucleus. It operates as part of the larger nuclear transport complex which relies on the Ran GTPase system for direction and timing. Importin 9's role is pivotal in regulating gene expression and cell cycle activities as transported molecules often influence transcriptional activity within the nucleus. This regulation assists in maintaining normal cellular functions and adaptations to stress signals.

Pathways

Importin 9 is integral to the nucleocytoplasmic transport pathway essential for several cellular processes including the regulation of signal transduction and chromatin remodeling. Ran GTPase facilitates the release of cargo once Importin 9 reaches the nucleus while the protein Importin β plays a cooperative role in many pathways. Together they ensure the correct localization of proteins necessary for DNA replication and repair highlighting Importin 9's role in DNA-related processes.

Dysfunction of Importin 9 links to neurological conditions and cancer. Abnormal regulation of nuclear transport may contribute to the pathogenesis of neurodegenerative diseases where Importin 9 collaborates with Tau protein in neuronal cells. In oncology altered Importin 9 activity has connections to tumorigenesis often involving the c-Myc protein where misregulated nuclear transport leads to uncontrolled cell proliferation and cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear transport receptor that mediates nuclear import of proteins, such as histones, proteasome and actin (PubMed : 11823430, PubMed : 30855230, PubMed : 34711951). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed : 11823430). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 11823430). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed : 11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 11823430). Mediates the import of pre-assembled proteasomes into the nucleus; AKIRIN2 acts as a molecular bridge between IPO9 and the proteasome complex (PubMed : 11823430, PubMed : 34711951). Mediates the nuclear import of histones H2A, H2B, H4 and H4 (PubMed : 11823430, PubMed : 30855230). In addition to nuclear import, also acts as a chaperone for histones by preventing inappropriate non-nucleosomal interactions (PubMed : 30855230). Mediates the nuclear import of actin (By similarity).
See full target information IPO9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Virus research 263:179-183 PubMed30769121

2019

Intracellular dynamics of actin affects Borna disease virus replication in the nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Hirai,Eisuke Domae,Yoshihiro Yoshikawa,Hideyuki Okamura,Akiko Makino,Keizo Tomonaga

iScience 9:314-327 PubMed30448731

2018

Nuclear Actin Polymerized by mDia2 Confines Centromere Movement during CENP-A Loading.

Applications

Unspecified application

Species

Unspecified reactive species

Chenshu Liu,Ruijun Zhu,Yinghui Mao
View all publications
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Product promise

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