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Rabbit Recombinant Monoclonal Importin 9/RANBP9 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] - BSA and Azide free (AB247974), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Expected
Mouse
Not recommended
Not recommended
Predicted
Expected
Rat
Not recommended
Not recommended
Predicted
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Target data

Function

Nuclear transport receptor that mediates nuclear import of proteins, such as histones, proteasome and actin (PubMed:11823430, PubMed:30855230, PubMed:34711951). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11823430). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11823430). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed:11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11823430). Mediates the import of pre-assembled proteasomes into the nucleus; AKIRIN2 acts as a molecular bridge between IPO9 and the proteasome complex (PubMed:11823430, PubMed:34711951). Mediates the nuclear import of histones H2A, H2B, H4 and H4 (PubMed:11823430, PubMed:30855230). In addition to nuclear import, also acts as a chaperone for histones by preventing inappropriate non-nucleosomal interactions (PubMed:30855230). Mediates the nuclear import of actin (By similarity).

Alternative names

IMP9, KIAA1192, RANBP9, HSPC273, IPO9, Importin-9, Imp9, Ran-binding protein 9, RanBP9

Recommended products

Rabbit Recombinant Monoclonal Importin 9/RANBP9 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR1352
Purification technique
Affinity purification Protein A
Dissociation constant
2.29 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247974 is the carrier-free version of Anti-Importin 9/RANBP9 antibody [EPR1352] ab124710.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Importin 9 also known as RANBP9 or IPO9 is a nuclear transport receptor with a molecular mass of approximately 107 kDa. This protein facilitates the transport of molecules into the nucleus a process essential for cellular function. Importin 9 is expressed in various tissues and plays a significant role in shuttling proteins and some RNA species across the nuclear envelope. As a member of the importin family it interacts with nuclear localization signals on target proteins steering them through nuclear pore complexes.

Biological function summary

Importin 9 participates in intracellular transport by binding cargo proteins in the cytoplasm and transporting them into the nucleus. It operates as part of the larger nuclear transport complex which relies on the Ran GTPase system for direction and timing. Importin 9's role is pivotal in regulating gene expression and cell cycle activities as transported molecules often influence transcriptional activity within the nucleus. This regulation assists in maintaining normal cellular functions and adaptations to stress signals.

Pathways

Importin 9 is integral to the nucleocytoplasmic transport pathway essential for several cellular processes including the regulation of signal transduction and chromatin remodeling. Ran GTPase facilitates the release of cargo once Importin 9 reaches the nucleus while the protein Importin β plays a cooperative role in many pathways. Together they ensure the correct localization of proteins necessary for DNA replication and repair highlighting Importin 9's role in DNA-related processes.

Associated diseases and disorders

Dysfunction of Importin 9 links to neurological conditions and cancer. Abnormal regulation of nuclear transport may contribute to the pathogenesis of neurodegenerative diseases where Importin 9 collaborates with Tau protein in neuronal cells. In oncology altered Importin 9 activity has connections to tumorigenesis often involving the c-Myc protein where misregulated nuclear transport leads to uncontrolled cell proliferation and cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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