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AB247974

Anti-Importin 9/RANBP9 antibody [EPR1352] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Importin 9/RANBP9 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

IMP9, KIAA1192, RANBP9, HSPC273, IPO9, Importin-9, Imp9, Ran-binding protein 9, RanBP9

1 Images
Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] - BSA and Azide free (AB247974)
  • WB

Supplier Data

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] - BSA and Azide free (AB247974)

This data was developed using the same antibody clone in a different buffer formulation (ab124710).

Lanes 1-3 : Merged signal (red and green). Green - ab124710. Red - loading control, ab8245 observed at 36 kDa.

ab124710 was shown to react with IPO9 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265352 (knockout cell lysate ab257483) was used. Wild-type and IPO9 knockout samples were subjected to SDS-PAGE. ab124710 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Importin 9/RANBP9 antibody [EPR1352] (<a href='/en-us/products/primary-antibodies/importin-9-ranbp9-antibody-epr1352-ab124710'>ab124710</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

IPO9 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human IPO9 (Importin 9/RANBP9) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ipo9-importin-9-ranbp9-knockout-hela-cell-line-ab265352'>ab265352</a>)

Lane 3:

SK-BR-3 cell lysate at 20 µg

Predicted band size: 116 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1352

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab247974 is the carrier-free version of ab124710.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Importin 9 also known as RANBP9 or IPO9 is a nuclear transport receptor with a molecular mass of approximately 107 kDa. This protein facilitates the transport of molecules into the nucleus a process essential for cellular function. Importin 9 is expressed in various tissues and plays a significant role in shuttling proteins and some RNA species across the nuclear envelope. As a member of the importin family it interacts with nuclear localization signals on target proteins steering them through nuclear pore complexes.
Biological function summary

Importin 9 participates in intracellular transport by binding cargo proteins in the cytoplasm and transporting them into the nucleus. It operates as part of the larger nuclear transport complex which relies on the Ran GTPase system for direction and timing. Importin 9's role is pivotal in regulating gene expression and cell cycle activities as transported molecules often influence transcriptional activity within the nucleus. This regulation assists in maintaining normal cellular functions and adaptations to stress signals.

Pathways

Importin 9 is integral to the nucleocytoplasmic transport pathway essential for several cellular processes including the regulation of signal transduction and chromatin remodeling. Ran GTPase facilitates the release of cargo once Importin 9 reaches the nucleus while the protein Importin β plays a cooperative role in many pathways. Together they ensure the correct localization of proteins necessary for DNA replication and repair highlighting Importin 9's role in DNA-related processes.

Dysfunction of Importin 9 links to neurological conditions and cancer. Abnormal regulation of nuclear transport may contribute to the pathogenesis of neurodegenerative diseases where Importin 9 collaborates with Tau protein in neuronal cells. In oncology altered Importin 9 activity has connections to tumorigenesis often involving the c-Myc protein where misregulated nuclear transport leads to uncontrolled cell proliferation and cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear transport receptor that mediates nuclear import of proteins, such as histones, proteasome and actin (PubMed : 11823430, PubMed : 30855230, PubMed : 34711951). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed : 11823430). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 11823430). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed : 11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 11823430). Mediates the import of pre-assembled proteasomes into the nucleus; AKIRIN2 acts as a molecular bridge between IPO9 and the proteasome complex (PubMed : 11823430, PubMed : 34711951). Mediates the nuclear import of histones H2A, H2B, H4 and H4 (PubMed : 11823430, PubMed : 30855230). In addition to nuclear import, also acts as a chaperone for histones by preventing inappropriate non-nucleosomal interactions (PubMed : 30855230). Mediates the nuclear import of actin (By similarity).
See full target information IPO9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 77:100019 PubMed35397366

2022

IRF4 suppresses osteogenic differentiation of BM-MSCs by transcriptionally activating miR-636/DOCK9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuepu Zhang,Yue Zhang,Limin Yang,Yuexin Wu,Xiaohu Ma,Gang Tong,Zhaoliang Ban,Haosen Zhao
View all publications
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