Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free

12 product images

• 

  Immunoprecipitation - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  Importin subunit alpha was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: HepG2 whole cell lysate
  
  Lane 2: Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 IP in HepG2 whole cell lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 in HepG2 whole cell lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 10 seconds.

  All lanes: Immunoprecipitation - Anti-Importin subunit alpha antibody [EPR26346-53] (Anti-Importin subunit alpha antibody [EPR26346-53] ab307438) at 1/1000 dilution

  All lanes: HepG2 whole cell lysate at 10 µg

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Importin subunit alpha was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: HepG2 whole cell lysate
  
  Lane 2: Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 IP in HepG2 whole cell lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 in HepG2 whole cell lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 10 seconds.

• 

  Flow Cytometry (Intracellular) - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Importin subunit alpha with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Importin subunit alpha with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/50 dilution (10.02 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
  
  Confocal image showing cytoplasmic and nuclear staining in untreated NIH/3T3 cells, and the signals were translocated to nucleus in NIH/3T3 cells after treatment with heat shock (45.5°C) for 1.5 h.
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

• 

  Flow Cytometry (Intracellular) - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Importin subunit alpha with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Importin subunit alpha with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/50 dilution (10.02 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
  
  Confocal image showing cytoplasmic and nuclear staining in untreated HeLa cells, and the signals were translocated to nucleus in HeLa cells after treatment with heat shock (45.5°C) for 1.5 h.
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  Immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma tissue labeling Importin subunit alpha with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/2000 dilution (0.251 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Positive staining on human colorectal adenocarcinoma.
  
  The section was incubated with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Importin subunit alpha with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/2000 dilution (0.251 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Positive staining on human breast cancer (PMID: 34709479).
  
  The section was incubated with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Importin subunit alpha with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 at 1/2000 dilution (0.251 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Positive staining on human breast (PMID: 34709479).
  
  The section was incubated with Anti-Importin subunit alpha antibody [EPR26346-53] ab307438 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Western blot - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  
  Exposure time: 39 seconds.

  All lanes: Western blot - Anti-Importin subunit alpha antibody [EPR26346-53] (Anti-Importin subunit alpha antibody [EPR26346-53] ab307438) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

  Lane 2: HeLa transfected with siRNA specifically targeting SRP1 whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 60 kDa

  Observed band size: 60 kDa

  Exposure time: 39s

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  
  Exposure time: 39 seconds.

• 

  Western blot - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 147 seconds.

  All lanes: Western blot - Anti-Importin subunit alpha antibody [EPR26346-53] (Anti-Importin subunit alpha antibody [EPR26346-53] ab307438) at 1/1000 dilution

  Lane 1: Human cerebellum tissue lysate at 20 µg

  Lane 2: Human heart tissue lysate at 20 µg

  Lane 3: Human kidney tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 60 kDa

  Observed band size: 60 kDa

  Exposure time: 147s

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 147 seconds.

• 

  Western blot - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 26 seconds.

  All lanes: Western blot - Anti-Importin subunit alpha antibody [EPR26346-53] (Anti-Importin subunit alpha antibody [EPR26346-53] ab307438) at 1/1000 dilution

  Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 3: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 60 kDa

  Observed band size: 60 kDa

  Exposure time: 26s

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 26 seconds.

• 

  Western blot - Anti-Importin subunit alpha antibody [EPR26346-53] - BSA and Azide free (ab307439)

  This data was produced using Anti-Importin subunit alpha antibody [EPR26346-53] ab307438, the same clone but in a different formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 37 seconds.

  All lanes: Western blot - Anti-Importin subunit alpha antibody [EPR26346-53] (Anti-Importin subunit alpha antibody [EPR26346-53] ab307438) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Rat brain tissue lysate at 20 µg

  Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 60 kDa

  Observed band size: 60 kDa

  Exposure time: 37s

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 37 seconds.