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Rabbit Recombinant Monoclonal Importin4/Imp4 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046), expandable thumbnail
  • Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat mediated antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR (PubMed:11823430, PubMed:16207705, PubMed:17682055, PubMed:21454524). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11823430, PubMed:16207705). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11823430, PubMed:16207705). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed:11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11823430). Mediates the nuclear import of the histone H3-H4 dimer when in complex with ASF1 (ASF1A or ASF1B) (PubMed:21454524, PubMed:29408485). Mediates the ligand-independent nuclear import of vitamin D receptor (VDR) (PubMed:16207705). In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS (PubMed:12610148).

Alternative names

IMP4B, RANBP4, IPO4, Importin-4, Imp4, Importin-4b, Ran-binding protein 4, Imp4b, RanBP4

Recommended products

Rabbit Recombinant Monoclonal Importin4/Imp4 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13660
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Importin4 (Imp4) also known as Ipo4 is a nuclear import receptor with an approximate mass of 130 kDa. This protein facilitates the transport of molecules such as ribosomal proteins and histones into the nucleus. Expression of Importin4 occurs in various tissues including the brain testis and liver. Importin4 plays a significant mechanical role by recognizing nuclear localization signals on cargo molecules and mediating their translocation across the nuclear envelope.

Biological function summary

Importin4 functions as part of the nuclear transport system a complex network essential for cellular operations. Importin4 interacts with the nuclear pore complex to enable import of specific protein cargo into the nucleus. By partnering with karyopherins and Ran-GTPase Importin4 participates in the active transport mechanism ensuring the correct localization of proteins that are critical to genomic regulation processes.

Pathways

Importin4 plays a role in the nuclear transport pathway and is associated with the ribosome biogenesis pathway. This protein works alongside proteins like Ran-GTPase enhancing the translocation of ribosomal components pivotal for cellular growth and protein synthesis. Importin4 also aids in the shuttling of histones which are essential to chromatin assembly and DNA transcription regulation.

Associated diseases and disorders

Importin4 has connections to cancer and neurodegenerative diseases. Altered expression and mutations in Importin4 may disrupt cellular homeostasis and nuclear transport linking it to tumorigenesis. Additionally associations with proteins such as karyopherin alpha importers indicate its involvement in pathological states affecting the nervous system potentially contributing to conditions such as Alzheimer's disease. Understanding Importin4's interactions could provide insights into these complex diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046), expandable thumbnail

    Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

    Lanes 1-4: Merged signal (red and green). Green - ab181046 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab181046 Anti-Importin4/Imp4 antibody [EPR13660] was shown to specifically react with Importin4/Imp4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human IPO4 (Importin4/Imp4) knockout HEK-293T cell line ab266175 (knockout cell lysate Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate ab258005) was used. Wild-type and Importin4/Imp4 knockout samples were subjected to SDS-PAGE. ab181046 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: IPO4 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell line (Human IPO4 (Importin4/Imp4) knockout HEK-293T cell line ab266175)

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: A549 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 119 kDa

    Observed band size: 130 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Importin4/Imp4 using ab181046 at a 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Importin4/Imp4 using ab181046 at a 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

    Immunofluorescent staining of A549 cells labeling Importin4/Imp4 using ab181046 at a 1/50 dilution (red) and DAPI nuclear staining (blue).

  • Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046), expandable thumbnail

    Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

    Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labelling Importin4/Imp4 immunoprecipitated using ab181046 at a 1/10 dilution.

    All lanes: Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

    Predicted band size: 119 kDa

  • Flow Cytometry (Intracellular) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

    Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling Importin4/Imp4 using ab181046 at a 1/10 dilution (red) or a rabbit IgG control (green).

  • Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046), expandable thumbnail

    Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)

    All lanes: Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/100000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: A549 cell lysate at 10 µg

    Lane 4: Human seminoma lysate at 10 µg

    Predicted band size: 119 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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