Anti-INCENP antibody [58-217]

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (AB23956)

ab23956 staining INCENP in HeLa cells, by immunofluorescence.

optimal antibody dilution : 1mg/ml

Image kindly submitted by Serena Orlando, Giulia Guarguaglini and Patrizia Lavia, from the University 'La Sapienza' CNR, Italy

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (AB23956)

ICC/IF image of ab23956 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23956, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INCENP antibody [58-217] (AB23956)

ab23956 staining human anal cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in a commercially available blocking agent for 20 minutes at 20°C. The primary anibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C. A HRP-conjugated goat anti-mouse antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.