AB55305

Anti-Indoleamine 2, 3-dioxygenase antibody [4D2]

Name: Anti-Indoleamine 2, 3-dioxygenase antibody [4D2] (ab55305) | Abcam
Brand: Abcam Limited
SKU: AB55305
Price: 715 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(40 Publications)

Mouse Monoclonal Indoleamine 2, 3-dioxygenase antibody. Suitable for Flow Cyt, WB, IHC-Fr and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IDO1.

View Alternative Names

IDO, INDO, IDO1, IDO-1

2 Images

Flow Cytometry - Anti-Indoleamine 2, 3-dioxygenase antibody [4D2] (AB55305)

Flow Cyt

Unknown

Flow Cytometry - Anti-Indoleamine 2, 3-dioxygenase antibody [4D2] (AB55305)

Overlay histogram showing HeLa cells stained with ab55305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55305, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Unknown

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [4D2] (AB55305)

Indoleamine 2 3-dioxygenase antibody (ab55305) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [4D2] (ab55305)

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4D2

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-Fr, Flow Cyt, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IDO1.

P14902

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

Product details

This product was changed from ascites to tissue culture supernatant on 30^th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.

Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174, PubMed : 18026683). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).

See full target information IDO1

style

left-column

Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Journal of hepatocellular carcinoma 10:921-934 PubMed37350801

2023

PD-L1 is Fascinating but IDO Needs Attention in Non-HCV and Non-HBV-Associated Hepatocellular Carcinoma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kashif Asghar,Shaarif Bashir,Iftikhar Ali Rana,Muhammad Abu Bakar,Asim Farooq,Muhammad Hassan,Zukhruf Asif,Mahnoor Afzal,Iqra Masood,Muhammad Ishaq,Muhammad Tahseen,Sundus Bilal,Shafqat Mehmood,Nosheen Kanwal,Islah Ud Din,Asif Loya

Asian Pacific journal of cancer prevention : APJCP 24:1591-1600 PubMed37247278

2023

High Indoleamine 2,3-Dioxygenase Expression along with Low Bridging Integrator-1 Expression in Hepatocellular Carcinoma Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kashif Asghar,Iftikhar Ali Rana,Muhammad Abubakar,Kinza Hasham,Muhammad Tahseen,Sundus Bilal,Shafqat Mehmood,Asim Farooq,Kashif Siddique,Asif Loya

BMC pregnancy and childbirth 22:983 PubMed36587196

2022

Molecular mechanisms involved in the IL-6-mediated upregulation of indoleamine 2,3-dioxygenase 1 (IDO1) expression in the chorionic villi and decidua of women in early pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Shuyun Zhao,Xiaojuan Chen,Ziwen Xiao,Xinghui Wen,Xingming Zhong,Shixiang Li,Hui Cheng,Guanyou Huang

JCI insight 7: PubMed35925682

2022

Immune cells and their inflammatory mediators modify β cells and cause checkpoint inhibitor-induced diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Luisa Perdigoto,Songyan Deng,Katherine C Du,Manik Kuchroo,Daniel B Burkhardt,Alexander Tong,Gary Israel,Marie E Robert,Stuart P Weisberg,Nancy Kirkiles-Smith,Angeliki M Stamatouli,Harriet M Kluger,Zoe Quandt,Arabella Young,Mei-Ling Yang,Mark J Mamula,Jordan S Pober,Mark S Anderson,Smita Krishnaswamy,Kevan C Herold

Cell death & disease 13:572 PubMed35760783

2022

IDO1 plays a tumor-promoting role via MDM2-mediated suppression of the p53 pathway in diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chengtao Sun,Mengzhen Li,Lian Zhang,Feifei Sun,Huimou Chen,Yanjie Xu,Yingxia Lan,Li Zhang,Suying Lu,Jia Zhu,Junting Huang,Juan Wang,Yang Hu,Yanfen Feng,Yizhuo Zhang

Cancers 14: PubMed35626147

2022

Mutational Activation of the NRF2 Pathway Upregulates Kynureninase Resulting in Tumor Immunosuppression and Poor Outcome in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes F Fahrmann,Ichidai Tanaka,Ehsan Irajizad,Xiangying Mao,Jennifer B Dennison,Eunice Murage,Julian Casabar,Jeffrey Mayo,Qian Peng,Muge Celiktas,Jody V Vykoukal,Soyoung Park,Ayumu Taguchi,Oliver Delgado,Satyendra C Tripathi,Hiroyuki Katayama,Luisa Maren Solis Soto,Jaime Rodriguez-Canales,Carmen Behrens,Ignacio Wistuba,Samir Hanash,Edwin J Ostrin

Experimental cell research 405:112677 PubMed34111474

2021

CD47 blockade enhances therapeutic efficacy of cisplatin against lung carcinoma in a murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilei Cui,Dengfei Xu,Fafu Zhang,Jinyuan Sun,Lin Song,Wenjing Ye,Junxiang Zeng,Min Zhou,Zhengshang Ruan,Linlin Zhang,Rongrong Ren

Cancer immunology research 9:707-722 PubMed33875483

2021

Targeting Triple-Negative Breast Cancer with Combination Therapy of EGFR CAR T Cells and CDK7 Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Xia,Zaozao Zheng,Jun-Yi Liu,Yu-Jie Chen,Jiancheng Ding,Guo-Sheng Hu,Ya-Hong Hu,Suling Liu,Wen-Xin Luo,Ning-Shao Xia,Wen Liu

World journal of clinical oncology 11:1018-1028 PubMed33437664

2021

Forkhead box P3 and indoleamine 2,3-dioxygenase co-expression in Pakistani triple negative breast cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kashif Asghar,Asif Loya,Iftikhar Ali Rana,Muhammad Abu Bakar,Asim Farooq,Muhammad Tahseen,Muhammad Ishaq,Iqra Masood,Muhammad Usman Rashid

RSC advances 10:45199-45206 PubMed35516257

2020

Design, synthesis and bioevaluation of novel 6-substituted aminoindazole derivatives as anticancer agents.

Applications

Unspecified application

Species

Unspecified reactive species

Ngo Xuan Hoang,Van-Hai Hoang,Thi-Thu-Trang Luu,Hung N Luu,Thien Ngo,Duong Van Hieu,Nguyen Huu Long,Le Viet Anh,Son Tung Ngo,Yen Thi Kim Nguyen,Byung Woo Han,Thanh Xuan Nguyen,Dinh Thi Thanh Hai,Tran Thi Thu Hien,Phuong-Thao Tran

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Indoleamine 2, 3-dioxygenase antibody [4D2]

Flow Cytometry - Anti-Indoleamine 2, 3-dioxygenase antibody [4D2] (AB55305)

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [4D2] (AB55305)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (40)

Product promise