AB134197

Anti-Indoleamine 2, 3-dioxygenase antibody

Name: Anti-Indoleamine 2, 3-dioxygenase antibody (ab134197) | Abcam
Brand: Abcam Limited
SKU: AB134197
Rating: 2 (3 reviews)

(3 Reviews)

(5 Publications)

Goat Polyclonal Indoleamine 2, 3-dioxygenase antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human IDO1 aa 100-150.

View Alternative Names

IDO, INDO, IDO1, IDO-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody (AB134197)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody (AB134197)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Indoleamine 2, 3-dioxygenase with ab134197 at 6 μg/ml dilution. Heat induced antigen retrieval with citrate buffer pH 6.

Supplier Data

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody (AB134197)

All lanes:

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody (ab134197) at 0.5 µg/mL

Lane 1:

Human lymph node lysate at 35 µg

Lane 2:

Spleen lysate at 35 µg

Lane 3:

Placenta lysate at 35 µg

Predicted band size: 45 kDa

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IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody (AB134197)

Negative control : Immunohistochemical analysis of paraffin-embedded Human placenta tissue with no primary antibody.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human IDO1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P14902

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Approx 50kDa band observed in Human Lymph Node, Spleen and Placenta lysates (calculated MW of 45.3kDa according to NP_002155.1).</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab134197 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.

Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174, PubMed : 18026683). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).

See full target information IDO1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of veterinary medical science 83:1885-1890 PubMed34690223

2021

Detection of indoleamine 2,3-dioxygenase 1-expressing cells in canine normal and tumor tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Namiko Ikeda,Daiki Kato,Masaya Tsuboi,Ryohei Yoshitake,Shotaro Eto,Sho Yoshimoto,Masahiro Shinada,Satoshi Kamoto,Yuko Hashimoto,Yousuke Takahashi,James Chambers,Kazuyuki Uchida,Ryohei Nishimura,Takayuki Nakagawa

Frontiers in immunology 11:622467 PubMed33552086

2021

GBP1 Facilitates Indoleamine 2,3-Dioxygenase Extracellular Secretion to Promote the Malignant Progression of Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yinnan Meng,Wei Wang,Meng Chen,Kuifei Chen,Xinhang Xia,Suna Zhou,Haihua Yang

Cell metabolism 27:667-676.e4 PubMed29514072

2018

Nicotinamide Improves Aspects of Healthspan, but Not Lifespan, in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah J Mitchell,Michel Bernier,Miguel A Aon,Sonia Cortassa,Eun Young Kim,Evandro F Fang,Hector H Palacios,Ahmed Ali,Ignacio Navas-Enamorado,Andrea Di Francesco,Tamzin A Kaiser,Tyler B Waltz,Ning Zhang,James L Ellis,Peter J Elliott,David W Frederick,Vilhelm A Bohr,Mark S Schmidt,Charles Brenner,David A Sinclair,Anthony A Sauve,Joseph A Baur,Rafael de Cabo

British journal of pharmacology 173:2352-68 PubMed27174364

2016

Hepatic NAD(+) deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Can-Can Zhou,Xi Yang,Xia Hua,Jian Liu,Mao-Bing Fan,Guo-Qiang Li,Jie Song,Tian-Ying Xu,Zhi-Yong Li,Yun-Feng Guan,Pei Wang,Chao-Yu Miao

PLoS pathogens 9:e1003341 PubMed23671415

2013

Differences in gastric carcinoma microenvironment stratify according to EBV infection intensity: implications for possible immune adjuvant therapy.

Applications

IHC-P

Species

Human

Michael J Strong,Guorong Xu,Joseph Coco,Carl Baribault,Dass S Vinay,Michelle R Lacey,Amy L Strong,Teresa A Lehman,Michael B Seddon,Zhen Lin,Monica Concha,Melody Baddoo,Marybeth Ferris,Kenneth F Swan,Deborah E Sullivan,Matthew E Burow,Christopher M Taylor,Erik K Flemington

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Indoleamine 2, 3-dioxygenase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody (AB134197)

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody (AB134197)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody (AB134197)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise