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Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IDO1.

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Images

Immunocytochemistry - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (AB243888), expandable thumbnail
  • Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (AB243888), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (AB243888), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (AB243888), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (AB243888), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human IDO1. Database link P14902

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCWB
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/500.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/500.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:14502282, PubMed:23103127, PubMed:25157255, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).

Alternative names

IDO, INDO, IDO1, IDO-1

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Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IDO1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human IDO1. Database link P14902
Clone number
BLR031F
Concentration
Loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.

Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

Associated diseases and disorders

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.

Product promise

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6 product images

  • Immunocytochemistry - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888), expandable thumbnail

    Immunocytochemistry - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

    Formalin-fixed, paraffin-embedded A549 (human lung carcinoma cell line) cells labeling Indoleamine 2,3-dioxygenase using ab243888 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. Untreated cells (left) and cells treated withhuman interferon-gamma (right) are shown. DAB staining.

  • Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888), expandable thumbnail

    Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

    Western blot analysis using ab243888 at 1/1000 dilution.

    Lane 1: HeLa (- IFN-gamma) whole cell lysate (50 μg).

    Lane 2: HeLa (+ IFN-gamma) whole cell lysate (50 μg).

    Lane 3: HEK-293T whole cell lysate (50 μg).

    Lane 4: HDLM-2 whole cell lysate (50 μg).

    Lane 5: HepG2 whole cell lysate (50 μg).

    Lane 6: HEK-293T (expressing IDO2) whole cell lysate (50 μg).

    Lane 7: HEK-293T (- IFN-gamma) whole cell lysate (50 μg).

    Lane 8: HEK-293T (expressing IDO1) whole cell lysate (50 μg).

    All lanes: Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for J130 using ab243888 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

    Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Indoleamine 2,3-dioxygenase using ab243888 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Indoleamine 2,3-dioxygenase using ab243888 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [BLR031F] (ab243888)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase using ab243888 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. Counterstain: DAPI (blue).

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Product protocols

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