Knockout Tested Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.
Images
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (AB76157), expandable thumbnail](https://content.abcam.com/products/images/indoleamine-2-3-dioxygenase-antibody-epr1230y-ab76157--western-blot-img165056.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (AB76157)")
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (AB76157), expandable thumbnail](https://content.abcam.com/products/images/indoleamine-2-3-dioxygenase-antibody-epr1230y-ab76157--western-blot-img77428.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (AB76157)")
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (AB76157), expandable thumbnail](https://content.abcam.com/products/images/indoleamine-2-3-dioxygenase-antibody-epr1230y-ab76157--western-blot-img433929.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (AB76157)")
Publications
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- Cell 172:423-438.e252017Intrinsic Immunity Shapes Viral Resistance of Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianfang Wu,Viet Loan Dao Thi,Yumin Huang,Eva Billerbeck,Debjani Saha,Hans-Heinrich Hoffmann,Yaomei Wang,Luis A Vale Silva,Stephanie Sarbanes,Tony Sun,Linda Andrus,Yingpu Yu,Corrine Quirk,Melody Li,Margaret R MacDonald,William M Schneider,Xiuli An,Brad R Rosenberg,Charles M RicePubMed 29249360
- SLAS discovery : advancing life sciences R & D 23:34-462017A High-Throughput Dose-Response Cellular Thermal Shift Assay for Rapid Screening of Drug Target Engagement in Living Cells, Exemplified Using SMYD3 and IDO1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDean E McNulty,William G Bonnette,Hongwei Qi,Liping Wang,Thau F Ho,Anna Waszkiewicz,Lorena A Kallal,Raman P Nagarajan,Melissa Stern,Amy M Quinn,Caretha L Creasy,Dai-Shi Su,Alan P Graves,Roland S Annan,Sharon M Sweitzer,Marc A HolbertPubMed 28957646
- The British journal of dermatology 177:809-8172017Immunotolerant indoleamine-2,3-dioxygenase is increased in condyloma acuminata.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZ Xie,M Zhang,W Xiong,H Y Wan,X C Zhao,T Xie,H Lei,Z C Lin,D S Luo,X L Liang,Y H ChenPubMed 28132413
- Stem cell research & therapy 8:1012017Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration.
- Oncotarget 7:57606-576172016Expression and regulation of immune-modulatory enzyme indoleamine 2,3-dioxygenase (IDO) by human airway epithelial cells and its effect on T cell activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWejdan A Aldajani,Fabián Salazar,Herb F Sewell,Alan Knox,Amir M GhaemmaghamiPubMed 27613847
- Journal of infection and chemotherapy : official j 20:460-42014Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-γ.
- Blood 116:1755-602010Tryptophan/kynurenine metabolism in human leukocytes is independent of superoxide and is fully maintained in chronic granulomatous disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuk See De Ravin et. al.PubMed 20511543
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
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1 product for Alternative Version
Target data
Function
Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:14502282, PubMed:23103127, PubMed:25157255, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).
Alternative names
IDO, INDO, IDO1, IDO-1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR1230Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.
- Biological function summary
IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.
- Pathways
IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.
- Associated diseases and disorders
IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157), expandable thumbnail](https://content.abcam.com/products/images/indoleamine-2-3-dioxygenase-antibody-epr1230y-ab76157--western-blot-img165056.jpg "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)")
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
Lanes 1 - 6: Merged signal (red and green). Green - ab76157 observed at 40 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.ab76157 was shown to react with Indoleamine 2, 3-dioxygenase in treated wild-type A549 cells in Western blot with loss of signal observed in treated IDO1 knockout cell line Human IDO1 (Indoleamine 2, 3-dioxygenase) knockout A549 cell line ab266949 (IDO1 knockout cell lysate ab256948). Wild-type A549 and IDO1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab76157 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 2500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/2500 dilution
Lane 1: Wild-type A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate at 20 µg
Lane 2: Wild-type A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate at 20 µg
Lane 2: Western blot - Human IDO1 (Indoleamine 2, 3-dioxygenase) knockout A549 cell line (Human IDO1 (Indoleamine 2, 3-dioxygenase) knockout A549 cell line ab266949)
Lane 3: IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate at 20 µg
Lane 4: IDO1 knockout A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate at 20 µg
Lane 5: SK-OV-3 cell lysate at 20 µg
Lane 6: MCF7 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 40 kDa
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157), expandable thumbnail](https://content.abcam.com/products/images/indoleamine-2-3-dioxygenase-antibody-epr1230y-ab76157--western-blot-img77428.jpg "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)")
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
The expression of Indoleamine 2, 3-dioxygenase can be upregulated by IFN-gamma (PMID: 29685162). We are unsure how to define the extra bands.
All lanes: Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157), expandable thumbnail](https://content.abcam.com/products/images/indoleamine-2-3-dioxygenase-antibody-epr1230y-ab76157--western-blot-img433929.jpg "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)")
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
Western blot: Anti-IDO1 antibody [EPR1230Y] (ab76157) staining at 1/2500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab76157 was shown to bind specifically to IDO1. A band was observed at 42/56 kDa in treated wild-type A549 cell lysates with no signal observed at this size in IDO1 knockout cell line. To generate this image, wild-type and IDO1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/2500 dilution
Lane 1: Wild-type A549 Treated IFN gamma (25 ng/mL, 48 h) ab281500 cell lysate at 20 µg
Lane 2: Wild-type A549 Vehicle Control IFN gamma (0 ng/mL, 48 h) ab255554 cell lysate at 20 µg
Lane 3: IDO1 knockout A549 Treated IFN gamma (25 ng/mL, 48 h) ab281489 cell lysate at 20 µg
Lane 4: IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/mL, 48 h) ab263515 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 42 kDa, 56 kDa
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