AB156787

Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3]

Name: Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787) | Abcam
Brand: Abcam Limited
SKU: AB156787
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

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(1 Review)

(5 Publications)

Mouse Monoclonal Indoleamine 2, 3-dioxygenase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IDO1.

View Alternative Names

IDO, INDO, IDO1, IDO-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.

Lab

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Lanes 1 - 6 : Merged signal (red and green). Green - ab156787 observed at 40 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab156787 was shown to react with Indoleamine 2, 3-dioxygenase in treated wild-type A549 cells in Western blot with no signal observed in treated IDO1 knockout cell line ab266949 (IDO1 knockout cell lysate ab256948). Wild-type A549 and IDO1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab156787 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 4000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787) at 1/4000 dilution

Lane 1:

Wild-type A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate at 20 µg

Lane 2:

Wild-type A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate at 20 µg

Lane 2:

Western blot - Human IDO1 (Indoleamine 2, 3-dioxygenase) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-ido1-indoleamine-2-3-dioxygenase-knockout-a549-cell-line-ab266949'>ab266949</a>)

Lane 3:

IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate at 20 µg

Lane 4:

IDO1 knockout A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate at 20 µg

Lane 5:

SK-OV-3 cell lysate at 20 µg

Lane 6:

MCF7 cell lysate at 20 µg

Predicted band size: 45 kDa

Observed band size: 40 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase, labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/100 dilution.

Supplier Data

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

All lanes:

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787) at 1/4000 dilution

Lane 1:

HEK293T cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase cDNA at 5 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1A3

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IDO1.

P14902

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.

Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174, PubMed : 18026683). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).

See full target information IDO1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer (Tokyo, Japan) 31:135-147 PubMed37981615

2023

IDO-1 impairs antitumor immunity of natural killer cells in triple-negative breast cancer via up-regulation of HLA-G.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Jing,Shukun Bai,Peipei Zhang,Hao Ren,Lintao Jia,Weimiao Li,Guoxu Zheng

International journal of general medicine 16:1713-1733 PubMed37187591

2023

IDO1 Promotes the Progression of NSCLC by Regulating the Polarization of M2 Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Chen,Jie Yao,Meng-Yu Zhang,Rui Li,Xiao Liu,Yi-Qing Qu

International journal of molecular sciences 24: PubMed36902242

2023

MUC1 Expression Affects the Immunoflogosis in Renal Cell Carcinoma Microenvironment through Complement System Activation and Immune Infiltrate Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Lucarelli,Giuseppe Stefano Netti,Monica Rutigliano,Francesco Lasorsa,Davide Loizzo,Martina Milella,Annalisa Schirinzi,Antonietta Fontana,Francesca Di Serio,Roberto Tamma,Domenico Ribatti,Michele Battaglia,Elena Ranieri,Pasquale Ditonno

Cell death & disease 13:572 PubMed35760783

2022

IDO1 plays a tumor-promoting role via MDM2-mediated suppression of the p53 pathway in diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chengtao Sun,Mengzhen Li,Lian Zhang,Feifei Sun,Huimou Chen,Yanjie Xu,Yingxia Lan,Li Zhang,Suying Lu,Jia Zhu,Junting Huang,Juan Wang,Yang Hu,Yanfen Feng,Yizhuo Zhang

Cancer research 76:818-30 PubMed26837767

2016

Hypoxia-Induced Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma Induces an Immunosuppressive Tumor Microenvironment to Promote Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Long-Yun Ye,Wei Chen,Xue-Li Bai,Xing-Yuan Xu,Qi Zhang,Xue-Feng Xia,Xu Sun,Guo-Gang Li,Qi-Da Hu,Qi-Han Fu,Ting-Bo Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (AB156787)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise