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Knockout Tested Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody. C-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (AB228468), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (AB228468), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (AB228468), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (AB228468), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (AB228468), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-PICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/400

Notes

Incubate for 30 minutes at 4°C.

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate for 10 minutes at room temperature.

Tested
Tested

Species

Human

Dilution info

1/15

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).

Alternative names

IDO-1, INDO, IDO, IDO1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody. C-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP260

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.

Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

Associated diseases and disorders

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.

Product promise

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11 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) treated with 50 ng/ml IFN-gamma for 16 h cells labeling Indoleamine 2, 3-dioxygenase with purified ab228468 at 1/15 (9.4μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Flow cytometry analysis of HeLa cells treated with 50ng/ml IFN-gamma for 16hours labeling Indoleamine 2, 3-dioxygenase with purified ab228468 at 1/200 dilution (0.705 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue.Untreated cells (Green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry (Intracellular) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Flow Cytometry analysis of IFN-gamma treated HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling Indoleamine 2, 3-dioxygenase with ab228468 1/400 dilution (green) compared to a negative control rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Formalin-fixed, paraffin-embedded human colon tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228468 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468), expandable thumbnail

    Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468)

    Western blot: Anti-IDO1 antibody [SP260] (ab228468) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab228468 was shown to bind specifically to IDO1. A band was observed at 42 kDa in treated wild-type A549 cell lysates with no signal observed at this size in IDO1 knockout cell line. To generate this image, wild-type and IDO1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [SP260] - C-terminal (ab228468) at 1/1000 dilution

    Lane 1: Wild-type A549 Treated IFN gamma (25 ng/mL, 48 h) ab281500 cell lysate at 20 µg

    Lane 2: Wild-type A549 Vehicle Control IFN gamma (0 ng/mL, 48 h) ab255554 cell lysate at 20 µg

    Lane 3: IDO1 knockout A549 Treated IFN gamma (25 ng/mL, 48 h) ab281489 cell lysate at 20 µg

    Lane 4: IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/mL, 48 h) ab263515 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 45 kDa

    Observed band size: 42 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com