AB238785

Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal Indoleamine 2, 3-dioxygenase antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IDO, INDO, IDO1, IDO-1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab228470).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228470).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228470).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228470).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) treated with IFN stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228470).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

Formalin-fixed, paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228470).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228470).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

Formalin-fixed, paraffin-embedded human lung tissue stained for Indoleamine 2, 3-dioxygenase with ab228470 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228470).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - BSA and Azide free (AB238785)

This data was developed using ab238785, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded wild type (top panels) or IDO1 knockout (lower panels) A549 cells (ab266949) labelling IDO1 with ab228470 at a concentration of 2.7µg/ml. Cells were pretreated with 25ng/ml interferon-gamma for 48hr prior to antibody incubation to induce target expression.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with a Bond^™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with NaCi buffer (pH 6.0, Epitope Retrieval Solution 1) for 20mins.

ab228470 Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal was incubated for 15mins at room temperature. Sections were counterstained with Hematoxylin. Images to the right show absence of staining with IgG isotype control antibody, rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Unconjugated

Anti-Indoleamine 2, 3-dioxygenase antibody [SP277] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP277

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Primary incubation for 10 minutes at room temperature.</p>" } } }

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Product details

ab238785 is the carrier-free version of ab228470.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.

Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174, PubMed : 18026683). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).

See full target information IDO1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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