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AB245555

Anti-INF2 antibody

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(1 Publication)

Rabbit Polyclonal INF2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human INF2 aa 1-50.

View Alternative Names

C14orf151, C14orf173, INF2, Inverted formin-2, HBEBP2-binding protein C

2 Images
Immunoprecipitation - Anti-INF2 antibody (AB245555)
  • IP

Supplier Data

Immunoprecipitation - Anti-INF2 antibody (AB245555)

INF2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab245555 used for IP at 6 μg/mg lysate. For WB 0.4 μg/ml.

Lane 1 : ab245555 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 10 seconds.

All lanes:

Immunoprecipitation - Anti-INF2 antibody (ab245555)

Predicted band size: 136 kDa

false

Western blot - Anti-INF2 antibody (AB245555)
  • WB

Supplier Data

Western blot - Anti-INF2 antibody (AB245555)

All lanes:

Western blot - Anti-INF2 antibody (ab245555) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigenadenocarcinoma) whole cell lysate at 50 µg

Predicted band size: 136 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human INF2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q27J81

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245555 was affinity purified using an epitope specific to INF2 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The INF2 protein officially named Inverted Formin 2 is involved in actin dynamics. It has a mass of approximately 139 kDa. INF2 exists throughout many tissues in the body with high expression in the kidney brain and heart. This protein structurally influences filamentous actin (F-actin) and globular actin (G-actin) balance facilitating cytoskeleton remodeling and cell shape changes. With these functions INF2 can take on different roles depending on cell type and physiological conditions.
Biological function summary

Inverted Formin 2 acts in multiple cellular processes like cell migration and division. It is a part of the formin family of proteins which are known to regulate actin filament polymerization. INF2 forms complexes with other proteins including mitochondria-associated membranes (MAMs). It connects the endoplasmic reticulum to mitochondria which supports cellular activities like calcium ion transfer and lipid biosynthesis.

Pathways

INF2 interacts with the Rho family GTPases in the regulation of actin cytoskeleton dynamics. It plays important roles in the RhoA pathway assisting the polymerization and depolymerization of actin filaments which impacts cell movement and morphology. INF2 interacts with diaphanous-related formins (DRFs) including mDia1 to support these cellular functions.

Abnormal regulation or mutation of INF2 can lead to serious conditions as shown in focal segmental glomerulosclerosis (FSGS) and Charcot-Marie-Tooth (CMT) disease. Mutations in INF2 causing defective protein interactions can trigger renal pathology by impairing the podocyte cytoskeleton. The connection with nephrin another protein critical for kidney function highlights further implications for FSGS. In neuromuscular disorders like CMT altered dynamics between INF2 and dynamin-related mitochondrial protein (DRP1) affect mitochondrial fission leading to axonal degeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Severs actin filaments and accelerates their polymerization and depolymerization.
See full target information INF2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular immunology 19:516-526 PubMed34983952

2022

Enhancing the HSV-1-mediated antitumor immune response by suppressing Bach1.

Applications

Unspecified application

Species

Unspecified reactive species

Chaohu Pan,Qiaomei Cai,Xiaorong Li,Lili Li,Liping Yang,Yu Chen,Junxiao Liu,Wancheng Liu,Meiling Gao,Tianqi Sui,Xiaoyang Wang,Huiming Fan,Jiayin Ruan,Yueyue Shi,Saihua Chen,Lucy S Cheng,Jiayong Liu,Heng Yang,Genhong Cheng
View all publications
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