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AB318987

Anti-influenza A H1N1 (A/Brisbane/59/2007) neuraminidase antibody [3A2] - BSA and Azide free

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Mouse Recombinant Monoclonal N1, influenza A H1N1, A/Brisbane/59/2007 antibody. Carrier free. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Influenza A virus (A/Brisbane/59/2007(H1N1)), Recombinant full length protein - Influenza A virus (A/Brisbane/59/2007(H1N1)) samples.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-influenza A H1N1 (A/Brisbane/59/2007) neuraminidase antibody [3A2] - BSA and Azide free (AB318987)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-influenza A H1N1 (A/Brisbane/59/2007) neuraminidase antibody [3A2] - BSA and Azide free (AB318987)

This data was developed using ab318986, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling influenza A H1N1 (A/Brisbane/59/2007) neuraminidase with ab318986 at 1/2000 (0.451 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a N1, H1N1, A/Brisbane/59/2007 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a N9, H7N9, A/Anhui/1/2013 or N1, H1N1, A/California/07/2009 vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : N1, H1N1, A/Brisbane/59/2007-Myc transfected cells
C : N1, H1N1, A/California/07/2009-Myc transfected cells
D : N9, H7N9, A/Anhui/1/2013-Myc transfected cells
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (10ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

Indirect ELISA - Anti-influenza A H1N1 (A/Brisbane/59/2007) neuraminidase antibody [3A2] - BSA and Azide free (AB318987)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-influenza A H1N1 (A/Brisbane/59/2007) neuraminidase antibody [3A2] - BSA and Azide free (AB318987)

This data was developed using ab318986, the same antibody clone in a different buffer formulation. Indirect ELISA analysis of ab318986 at 2 ug/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution. Antigen : N1,influenza A H1N1, A/Brisbane/59/2007. Antigen concentration : 0-1000 ug/ml.

  • Unconjugated

    Anti-influenza A H1N1 (A/Brisbane/59/2007) neuraminidase antibody [3A2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A2

Isotype

IgG2a

Carrier free

Yes

Reacts with

Influenza A virus (A/Brisbane/59/2007(H1N1))

Applications

ICC/IF, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with Influenza A virus H1N1, A/Brisbane/59/2007 neuraminidase and does not react with Influenza A virus H1N1, A/California/07/2009 neuraminidase or Influenza A virus H7N9, A/Anhui/1/2013 neuraminidase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/Brisbane/59/2007(H1N1))": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Influenza A virus (A/Brisbane/59/2007(H1N1))": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Influenza A virus (A/Brisbane/59/2007(H1N1))": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab318987 is the carrirer-free version of ab318986.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A H1N1 (A/Brisbane/59/2007) neuraminidase also known as NA plays an important role mechanically in the viral life cycle. This enzyme facilitates the release of viral particles from infected host cells by cleaving sialic acid residues from glycoproteins. NA has an approximate molecular weight of 60 kDa and is expressed on the surface of influenza viral particles. Neuraminidase works closely with hemagglutinin (HA) another surface glycoprotein to ensure efficient viral budding and spread.
Biological function summary

Influenza A H1N1 neuraminidase contributes significantly to the virus's replication and transmission. As part of the viral envelope NA aids in the detachment of newly formed virions from the host cell surface. It operates as a homotetramer meaning it forms a complex with four identical subunits to execute its enzymatic activity. The neuraminidase protein is critical for overcoming the host's mucosal barriers facilitating infection of respiratory epithelial cells.

Pathways

Neuraminidase from the H1N1 influenza strain is directly involved in the viral replication cycle. The two main pathways where NA functions are the influenza virus life cycle and viral entry into host cells. In these pathways neuraminidase acts in tandem with hemagglutinin (HA) to mediate the initial host cell entry and subsequent virus spread making it a focal point for vaccine and antiviral drug development.

Influenza A H1N1 neuraminidase associates closely with influenza infection a significant cause of respiratory illness globally. The NA protein also plays a role in the pathogenesis of pandemic influenza marked by rapid spread and high morbidity. In this context neuraminidase inhibitors such as oseltamivir target NA to disrupt its function and curb the infection. Additionally research connects neural cell adhesion molecule (NCAM) to influenza pathogenesis highlighting the complex interactions between viral proteins and host cell receptors that contribute to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N1, influenza A H1N1, A/Brisbane/59/2007
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com