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AB318989

Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] - BSA and Azide free

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Mouse Recombinant Monoclonal N1, influenza H1N1, A/California/07/2009 antibody. Carrier free. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Influenza A virus (A/California/07/2009(H1N1)), Recombinant full length protein - Influenza A virus (A/California/07/2009(H1N1)) samples.

View Alternative Names

C3W6G3, C3W6G3_9INFA, Neuraminidase, N1, influenza H1N1, A/California/07/2009

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] - BSA and Azide free (AB318989)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] - BSA and Azide free (AB318989)

This data was developed using ab318988, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling influenza A H1N1 (A/California/07/2009) neuraminidase with ab318988 at 1/2000 (0.465 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a N1, H1N1, A/California/07/2009 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a N9, H7N9, A/Anhui/1/2013 or N1, H1N1, A/Brisbane/59/2007 vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : N1, H1N1, A/Brisbane/59/2007-Myc transfected cells
C : N1, H1N1, A/California/07/2009-Myc transfected cells
D : N9, H7N9, A/Anhui/1/2013-Myc transfected cells
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2ug/ml) dilution.

Indirect ELISA - Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] - BSA and Azide free (AB318989)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] - BSA and Azide free (AB318989)

This data was developed using ab318988, the same antibody clone in a different buffer formulation. Indirect ELISA analysis of ab318988 at 2000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution. Antigen : N1, influenza H1N1, A/California/07/2009. Antigen concentration : 0-1000 ug/ml.

  • Unconjugated

    Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CD6

Isotype

IgG2a

Carrier free

Yes

Reacts with

Influenza A virus (A/California/07/2009(H1N1))

Applications

I-ELISA, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with Influenza A virus H1N1, A/California/07/2009 neuraminidase and does not react with Influenza A virus H1N1, A/Brisbane/59/2007 neuraminidase or Influenza A virus H7N9, A/Anhui/1/2013 neuraminidase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/California/07/2009(H1N1))": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Influenza A virus (A/California/07/2009(H1N1))": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Influenza A virus (A/California/07/2009(H1N1))": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab318989 is the carrirer-free version of ab318988.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A H1N1 (A/California/07/2009) neuraminidase also known as NA is an enzyme important for the viral life cycle. It cleaves sialic acid groups from glycoproteins facilitating the release of new virus particles from host cells. This process aids the spread of infection within the host. Neuraminidase of H1N1 has a molecular mass of around 60 kDa. It is expressed on the surface of the influenza virus where it functions to support the viral budding process.
Biological function summary

Neuraminidase enables the influenza virus to exit infected cells and invade new ones ensuring efficient viral dissemination. It functions as part of a complex with the viral hemagglutinin protein which helps the virus attach to the host cell surface before infection. Neuraminidase's enzymatic action counteracts hemagglutinin's binding by cutting sialic acid residues from host receptors an essential function for viral replication.

Pathways

Neuraminidase plays a role in the influenza viral budding and release pathway coordinating with hemagglutinin to manage the balance between virus attachment and release. This action relates to various antiviral research efforts aimed at blocking this process focusing on the hemagglutinin-neuraminidase balance. Interventions at this level may inhibit the virus' ability to proliferate highlighting the importance of neuraminidase in therapeutic pathways targeting influenza.

Neuraminidase is directly linked to influenza infection a respiratory illness with pandemic potential. Inhibitors like oseltamivir and zanamivir are designed to block neuraminidase activity preventing the virus' spread in the body. These inhibitors target the interaction between neuraminidase and sialic acid residues aiming to disrupt the pathogenesis of the influenza virus. This interaction offers a promising target for therapeutic strategies against influenza virus strains including potential drug-resistant variants.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N1, influenza H1N1, A/California/07/2009
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com