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AB281949

Anti-Influenza A H1N1 hemagglutinin antibody [10]

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(1 Publication)

Rabbit Recombinant Monoclonal Hemagglutinin antibody. Carrier free. Suitable for WB and reacts with Influenza A virus (A/California/14/2009(H1N1)) samples. Cited in 1 publication.

View Alternative Names

Hemagglutinin, HA

1 Images
Western blot - Anti-Influenza A H1N1 hemagglutinin antibody [10] (AB281949)
  • WB

Supplier Data

Western blot - Anti-Influenza A H1N1 hemagglutinin antibody [10] (AB281949)

Performed under reducing conditions.

All lanes:

Western blot - Anti-Influenza A H1N1 hemagglutinin antibody [10] (ab281949) at 1/1000 dilution

Lane 1:

Influenza A H1N1 (A/California/04/2009) Hemagglutinin / HA at 0.5 µg

Lane 2:

Influenza A H1N1 (A/California/04/2009) Hemagglutinin / HA at 0.1 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

10

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A virus (A/California/04/2009(H1N1))

Applications

WB

applications

Specificity

**Specificity**H1N1 Hemagglutinin/HA **No cross-reactivity in ELISA with** H1N1 (A/Brisbane/59/2007) HA H1N1 (A/Brevig Mission/1/1918) HA H5N1 (A/Anhui/1/2005) HA H5N1 (A/Anhui/1/2005) HA2 Sub-unit H5N1 (A/turkey/Turkey/1/2005) HA H5N1 (A/bar-headed goose/Qinghai/14/2008) HA H5N1 (A/Indonesia/5/2005) HA H5N1 (A/Viet Nam/1203/2004) HA H3N2 (A/Brisbane/10/2007) HA Influenza B (B/Florida/4/2006) HA

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/California/04/2009(H1N1))": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Influenza A virus (A/California/14/2009(H1N1))": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A H1N1 hemagglutinin commonly referred to as H1N1 hemagglutinin is a glycoprotein found on the surface of the influenza virus. This protein with a mass of about 76 kDa enables the virus to attach and fuse with host cells in the respiratory tract. Hemagglutinin is expressed on the viral envelope allowing it to bind to sialic acid receptors on host cells facilitating viral entry through endocytosis. It is a critical component of the viral life cycle and an important target for immune response.
Biological function summary

The H1N1 hemagglutinin plays a significant role in viral infectivity and spread. It exists as a homotrimer complex on the virus surface which undergoes conformational changes to mediate membrane fusion. This protein is also a major antigen that elicits immune responses leading to the production of H1N1 flu antibodies. Importantly the antigenic properties of hemagglutinin drive the need for seasonal flu vaccines as variations in its structure such as those seen in the H1N1 Brisbane strain can affect vaccine efficacy.

Pathways

H1N1 hemagglutinin functions within the viral entry and replication pathways. During infection it works alongside neuraminidase another surface protein to allow proper viral release from infected cells. The interaction between hemagglutinin and cellular sialic acids is important for initiating the viral replication cycle. These interactions mark the beginning of the RIG-I signaling pathway which triggers innate immune responses against the virus.

H1N1 hemagglutinin is linked directly to influenza A infections such as the H1N1 flu which can lead to severe respiratory illness. This protein's variability is associated with pandemics highlighting its importance in disease spread and immune evasion. Changes in the hemagglutinin structure such as those observed in the H1N1 swine flu can cause shifts in virus infectivity. Additionally hemagglutinin interacts with host proteins during infection including pattern recognition receptors which detect viral components and initiate immune defense mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
See full target information HA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 16:e0073125 PubMed40626651

2025

Pathogenicity, transmissibility, and receptor binding of a human-isolated influenza A (H10N5) virus.

Applications

Unspecified application

Species

Unspecified reactive species

Mengchan Hao,Jiaying Wu,Lina Ji,Yubo Zhao,Shunyuan Zhang,Yiwei Guan,Liangyu Li,Wenxue Yang,Yuan Zhang,Jianjun Chen
View all publications
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