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AB276074

Anti-Influenza A H1N1 hemagglutinin antibody [9G1G8] - BSA and Azide free

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Mouse Monoclonal Hemagglutinin antibody. Carrier free. Suitable for Flow Cyt and reacts with Influenza A samples.

View Alternative Names

Hemagglutinin, HA

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Flow Cytometry - Anti-Influenza A H1N1 hemagglutinin antibody [9G1G8] - BSA and Azide free (AB276074)
  • Flow Cyt

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Flow Cytometry - Anti-Influenza A H1N1 hemagglutinin antibody [9G1G8] - BSA and Azide free (AB276074)

Flow cytometric analysis of Bac-HA infected High Five™ (clonal isolate derived from the parental Trichoplusia ni cell line) cells labeling hemagglutinin (HA) with 1 μg ab276074 (Black line) compared with a negative isotype control (Grey line), followed by 1 μg of FITC Goat Anti-Mouse Ig secondary antibody.

High Five™ cells were collected at 48 hours postinfection, and 106 cells were stained with ab276074 for 20 minutes on ice. Cells were washed twice and incubated with secondary antibody for 20 minutes on ice. Cells were washed twice and analyzed by flow cytometry.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9G1G8

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A

Applications

Flow Cyt

applications

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/25 - 1/100", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A H1N1 hemagglutinin commonly referred to as H1N1 hemagglutinin is a glycoprotein found on the surface of the influenza virus. This protein with a mass of about 76 kDa enables the virus to attach and fuse with host cells in the respiratory tract. Hemagglutinin is expressed on the viral envelope allowing it to bind to sialic acid receptors on host cells facilitating viral entry through endocytosis. It is a critical component of the viral life cycle and an important target for immune response.
Biological function summary

The H1N1 hemagglutinin plays a significant role in viral infectivity and spread. It exists as a homotrimer complex on the virus surface which undergoes conformational changes to mediate membrane fusion. This protein is also a major antigen that elicits immune responses leading to the production of H1N1 flu antibodies. Importantly the antigenic properties of hemagglutinin drive the need for seasonal flu vaccines as variations in its structure such as those seen in the H1N1 Brisbane strain can affect vaccine efficacy.

Pathways

H1N1 hemagglutinin functions within the viral entry and replication pathways. During infection it works alongside neuraminidase another surface protein to allow proper viral release from infected cells. The interaction between hemagglutinin and cellular sialic acids is important for initiating the viral replication cycle. These interactions mark the beginning of the RIG-I signaling pathway which triggers innate immune responses against the virus.

H1N1 hemagglutinin is linked directly to influenza A infections such as the H1N1 flu which can lead to severe respiratory illness. This protein's variability is associated with pandemics highlighting its importance in disease spread and immune evasion. Changes in the hemagglutinin structure such as those observed in the H1N1 swine flu can cause shifts in virus infectivity. Additionally hemagglutinin interacts with host proteins during infection including pattern recognition receptors which detect viral components and initiate immune defense mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
See full target information HA
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