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AB302938

Anti-Influenza A H7N9 Neuraminidase antibody [10F4]

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Mouse Recombinant Monoclonal Influenza A H7N9 Neuraminidase antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Influenza A, Influenza A samples.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] (AB302938)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] (AB302938)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney epithelial cell) cells labeling Influenza A H7N9 Neuraminidase with ab302938 at 1/50 dilution (21.86 µg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2µg/mL) (Green). Confocal image showing membrane staining in HEK-293T cells transfected with a mouse N9, H7N9, A/Anhui/1/2013 expression vector containing a myc tag. Anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (5µg/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2µg/mL).

Indirect ELISA - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] (AB302938)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] (AB302938)

Indirect ELISA - Anti-Influenza A H7N9 Neuraminidase antibody [10F4] (ab302938).

Primary antibody used at 0-2000 ng/ml.

Antigen concentration 5000 ng/ml.

Secondary antibody is Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.

  • Carrier free

    Anti-Influenza A H7N9 Neuraminidase antibody [10F4] - BSA and Azide Free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10F4

Isotype

IgG1

Carrier free

No

Reacts with

Influenza A

Applications

ICC/IF, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Use at 250 ng/ml.</p>" }, "Transfected cell line - Influenza A": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A H7N9 Neuraminidase also known as NA or sialidase is an enzyme important in the viral replication process of the influenza virus. This protein with a molecular mass of approximately 47 kDa is expressed on the surface of the influenza A virus particles. Neuraminidase facilitates the removal of sialic acid residues from glycoproteins and glycolipids which is essential for the release of new viral particles from infected host cells. The enzymatic action is an important factor in the virus's spread within the respiratory tract of the host organism.
Biological function summary

The action of Influenza A H7N9 Neuraminidase assists in the completion of the viral life cycle. Neuraminidase in conjunction with hemagglutinin is part of a complex that allows the virus to attach and penetrate host cells efficiently. By cleaving sialic acid residues neuraminidase enables the newly formed viral particles to detach and spread facilitating infection progression. This protein's ability to disrupt cellular interactions highlights its role in viral pathogenicity an essential feature for the influenza virus's survival in host organisms.

Pathways

Neuraminidase plays a significant part in the influenza viral entry and propagation pathways. Its interaction with hemagglutinin is important for the entry of the virus into host cells. Neuraminidase's activity supports the recycling of viral particles therefore contributing to the greater viral lifecycle network. Additionally its function links with pathways of immune evasion where it helps in avoiding the host's immune response primarily through altering host cell surface molecules and aiding in the spread of infection.

Neuraminidase is closely associated with the pathogenesis of influenza particularly with influenza A and its subtypes such as H7N9. Neuraminidase inhibitors like oseltamivir target this protein to prevent the spread of influenza virus and mitigate symptoms. Resistance to such inhibitors can arise making the study of neuraminidase important for developing effective treatments. Neuraminidase's activity affects both the viral lifecycle and the host's immune response underlining its role in the interactions between influenza virus proteins and host immune components.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Influenza A H7N9 Neuraminidase
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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