JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308171

Anti-Influenza A virus H10N3 HA (Hemagglutinin) antibody [HL1559]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Hemagglutinin antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Transfected cell line - Influenza A samples.

View Alternative Names

HA, HA2, Hemagglutinin

2 Images
Immunohistochemistry paraffin embedded sections - Anti-Influenza A virus H10N3 HA (Hemagglutinin) antibody [HL1559] (AB308171)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Influenza A virus H10N3 HA (Hemagglutinin) antibody [HL1559] (AB308171)

Immunohistochemical analysis of paraffin-embedded mock and Avian Influenza A virus H10N3 HA - transfected 293T cell pellets labeling Avian Influenza A virus H10N3 HA (Hemagglutinin) protein with ab308171 at 1/1000 dilution (green). Cytoplasm staining on HEK-293T is observed. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins. Nuclear DNA was labeled with Fluoroshield with DAPI (blue).

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus H10N3 HA (Hemagglutinin) antibody [HL1559] (AB308171)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus H10N3 HA (Hemagglutinin) antibody [HL1559] (AB308171)

For immunofluorescence analysis, mock (left panels) and transfected (right panels) HEK-293T cells were fixed in 4% paraformaldehyde at room temperature for 15 min and permeabilized for detection of Influenza A virus H10N3 HA (Hemagglutinin) protein using ab308171 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1559

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A

Applications

IHC-P, ICC/IF

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Influenza A": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A Hemagglutinin often called hemagglutinin or the HA protein is a glycoprotein found on the surface of the Influenza A virus. It plays a mechanical role in the viral entry by binding to sialic acid residues on host cells. This binding allows the virus to attach and initiate infection. Hemagglutinin is known for its significant molecular mass of approximately 75 kDa. It expresses in various strains of the Influenza A virus including the Hong Kong strain and A/California/07/2009. It is an important component in determining the virus's ability to infect different host species.
Biological function summary

The HA protein functions in a critical step of the viral life cycle called fusion. During the infection process hemagglutinin undergoes a structural change that facilitates the fusion of the viral envelope with the host cell membrane. This fusion process is essential to release the viral genome into the host cell. Hemagglutinin generally operates as part of a trimeric complex which enhances its ability to interact with host cell receptors. This interaction is vital for the virus to efficiently invade the host cells and propagate the infection throughout the host.

Pathways

Hemagglutinin plays an important role in the viral fusion pathway and the innate immune response pathway. It interacts with proteins like neuraminidase another surface protein of the influenza virus which assists in the release of progeny viruses from infected cells. During the fusion pathway HA's structural changes allow the viral and cellular membranes to merge a step necessary for the virus to establish infection. Its involvement in the immune response pathway also prompts the host immune system to recognize and respond to viral threats.

Hemagglutinin is closely associated with influenza a contagious respiratory illness caused by influenza viruses. Mutations or antigenic shifts in HA contribute to seasonal flu epidemics and pandemics. The HA protein is a significant target for antibodies because its alteration can lead to vaccine inefficacy. Additionally it is associated with immune escape mechanisms where structural changes reduce antibody recognition. Researchers often study the interaction of hemagglutinin and antibodies like those targeting neuraminidase as these interactions are important for developing effective vaccines and antiviral therapies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Influenza A Virus Hemagglutinin
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB205606

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB205606)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com