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Human Monoclonal Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2)), Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2)) samples.

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Images

Indirect ELISA - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (AB322519), expandable thumbnail
  • Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (AB322519), expandable thumbnail

Key facts

Isotype
IgG
Host species
Human
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBI-ELISA
Influenza A virus (A/Texas/50/2012(H3N2))
Predicted
Predicted
Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2))
Not recommended
Tested
Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2))
Tested
Not recommended

Tested
Tested

Species
Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2))
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Influenza A virus (A/Texas/50/2012(H3N2))
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2))
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2))
Dilution info
62.5 ng/mL
Notes

-

Predicted
Predicted

Species
Influenza A virus (A/Texas/50/2012(H3N2))
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2))
Dilution info
-
Notes

-

Target data

Human Monoclonal Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2)), Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2)) samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Indirect ELISA - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (ab322519), expandable thumbnail

    Indirect ELISA - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (ab322519)

    Indirect ELISA analysis of ab322519 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1:1000 dilution.

    Antigen: Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin / HA Protein.

    Antigen concentration: 1000 ng/ml

  • Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (ab322519), expandable thumbnail

    Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (ab322519)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (ab322519) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: 293T transfected with a Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 3: 293T transfected with a Influenza A Virus H1N1 (A/California/04/2009) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 4: 293T transfected with a Influenza A Virus H5N1 (strain A/Vietnam/1203/2004 H5N1) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (Goat Anti-Human IgG H&L (HRP) ab6858) at 1/10000 dilution

    Observed band size: 75 kDa, 36 kDa

    Exposure time: 4s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com