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AB322519

Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29]

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Human Recombinant Monoclonal Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2)), Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2)) samples.
2 Images
Indirect ELISA - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (AB322519)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (AB322519)

Indirect ELISA analysis of ab322519 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin / HA Protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (AB322519)
  • WB

Supplier Data

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (AB322519)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (ab322519) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with a Influenza A Virus H1N1 (A/California/04/2009) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with a Influenza A Virus H5N1 (strain A/Vietnam/1203/2004 H5N1) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/10000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 4s

  • Carrier free

    Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] - BSA and Azide free

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

S5V2-29

Isotype

IgG

Carrier free

No

Reacts with

Influenza A virus (A/Texas/50/2012(H3N2))

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/Texas/50/2012(H3N2))": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2))": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "62.5 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2))": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com