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AB324827

Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] - BSA and Azide free

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Human Recombinant Monoclonal Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2)), Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2)) samples.
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Indirect ELISA - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] - BSA and Azide free (AB324827)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] - BSA and Azide free (AB324827)

This data was developed using ab322519, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322519 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin / HA Protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] - BSA and Azide free (AB324827)
  • WB

Supplier Data

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] - BSA and Azide free (AB324827)

This data was developed using ab322519, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (<a href='/en-us/products/primary-antibodies/influenza-a-virus-h3n2-a-texas-50-2012-hemagglutinin-antibody-s5v2-29-ab322519'>ab322519</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with a Influenza A Virus H1N1 (A/California/04/2009) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with a Influenza A Virus H5N1 (strain A/Vietnam/1203/2004 H5N1) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/10000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 4s

  • Unconjugated

    Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29]

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

S5V2-29

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A virus (A/Texas/50/2012(H3N2))

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A virus (A/Texas/50/2012(H3N2))": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Influenza A virus (A/Texas/50/2012(H3N2))": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Influenza A virus (A/Texas/50/2012(H3N2))": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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