Rabbit Recombinant Monoclonal M1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - Influenza A, Influenza A, Transfected cell lysate - Influenza A samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (AB308354), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-m1-matrix-protein-antibody-hl1273-ab308354--immunocytochemistry-immunofluorescence-img408056.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (AB308354)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (AB308354), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-m1-matrix-protein-antibody-hl1273-ab308354--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408055.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (AB308354)")
![Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (AB308354), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-m1-matrix-protein-antibody-hl1273-ab308354--western-blot-img408052.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (AB308354)")
![Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (AB308354), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-m1-matrix-protein-antibody-hl1273-ab308354--western-blot-img408036.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (AB308354)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Influenza A | Expected | Tested | Tested |
| Transfected cell line - Influenza A | Tested | Not recommended | Not recommended |
| Transfected cell lysate - Influenza A | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Influenza A | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Influenza A | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A | Dilution info 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Influenza A, Transfected cell lysate - Influenza A | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Influenza A | Dilution info 1/5000 | Notes - |
Species Influenza A | Dilution info 1/5000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Influenza A | Dilution info - | Notes - |
Target data
Alternative names
Matrix Protein 1, Matrix Protein I, Membrane matrix protein M1
Recommended products
Rabbit Recombinant Monoclonal M1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - Influenza A, Influenza A, Transfected cell lysate - Influenza A samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1273
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-m1-matrix-protein-antibody-hl1273-ab308354--immunocytochemistry-immunofluorescence-img408056.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354)")
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354)
For immunofluorescence analysis, Influenza A and B Virus infected cells slide were permeabilized for detection of Influenza A virus M1 matrix protein using ab308354 at 1/100 dilution (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-m1-matrix-protein-antibody-hl1273-ab308354--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408055.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354)
Immunohistochemical analysis of Influenza A virus M1 transfected HEK293T cell pellet labeling Influenza A virus M1 matrix protein using ab308354 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-m1-matrix-protein-antibody-hl1273-ab308354--western-blot-img408052.jpg "Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354)")
Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus M1 (matrix protein) antibody [HL1273] (ab308354) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354) at 1/5000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-m1-matrix-protein-antibody-hl1273-ab308354--western-blot-img408036.jpg "Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354)")
Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354)
Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus M1 (matrix protein) antibody [HL1273] (ab308354) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Influenza A virus M1 matrix protein antibody [HL1273] (ab308354) at 1/5000 dilution
Lane 1: Non-infected (–) H1299 whole cell extracts at 15 µg
Lane 2: Infected (+) H1299 whole cell extracts at 15 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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