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AB308200

Anti-Influenza A virus M2 (matrix protein) antibody [HL1856]

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Rabbit Recombinant Monoclonal M2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Influenza A samples.

View Alternative Names

Influenza A virus matrix protein M2, Matrix protein 2, Membrane ion channel M2, Membrane protein M2, Proton channel protein M2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus M2 (matrix protein) antibody [HL1856] (AB308200)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus M2 (matrix protein) antibody [HL1856] (AB308200)

For immunofluorescence analysis, mock and transfected HEK-293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Influenza A virus M2 (matrix protein) protein using ab308200 at 1/500 dilution (green)

Nuclei (blue) is stained with fluoroshield with DAPI

Western blot - Anti-Influenza A virus M2 (matrix protein) antibody [HL1856] (AB308200)
  • WB

Supplier Data

Western blot - Anti-Influenza A virus M2 (matrix protein) antibody [HL1856] (AB308200)

Non-infected (–) and infected (+) H1299 whole cell extract (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody [HL1856] (ab308200) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-Influenza A virus M2 (matrix protein) antibody [HL1856] (ab308200) at 1/5000 dilution

Lane 1:

Non-infected (–) H1299 whole cell lysate at 15 µg

Lane 2:

Infected (+) H1299 whole cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-Influenza A virus M2 (matrix protein) antibody [HL1856] (AB308200)
  • WB

Supplier Data

Western blot - Anti-Influenza A virus M2 (matrix protein) antibody [HL1856] (AB308200)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody [HL1856] (ab308200) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Influenza A virus M2 (matrix protein) antibody [HL1856] (ab308200) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK-293T whole cell lysate at 30 µg

Lane 2:

Transfected (+) HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1856

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Influenza A": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Influenza A Virus M2 Protein also known as M2 ion channel or matrix protein 2 plays an essential role in the viral life cycle. This protein has a mass of approximately 97 amino acids and is expressed on the envelope of the Influenza A virus. It functions as a proton-selective channel facilitating the acidification of the viral interior after the virus enters the host cell. This acidification process is important for the uncoating of the viral genome allowing subsequent replication of the virus in host cells.
Biological function summary

The M2 protein from Influenza A virus participates in virus assembly budding and release. The protein operates as a tetramer creating a channel across the viral membrane. This channel is essential for the correct function and structure of the virus playing an important role in membrane fusion and release of the viral particles. It often works in conjunction with another matrix protein M1 which assists in stabilizing the integrity of the viral structure.

Pathways

The M2 Influenza protein is integral to the viral replication and assembly pathway. It aligns with the function of the viral ribonucleoprotein complex and is significant in the viral entry and exit pathways. Additionally the connection to the M1 matrix protein highlights its coordinated role in facilitating the assembly and release of new virions from the infected cells emphasizing its necessity in productive viral replication.

The Influenza A virus M2 protein is directly related to the pathogenesis of seasonal influenza. Antiviral drugs like amantadine and rimantadine target this ion channel to disrupt viral replication. Resistance mutations in the M2 protein can influence the efficacy of these drugs impacting treatment outcomes. There is also a link with immune system responses where M2 interactions with other viral proteins can modulate host immune evasion mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Influenza A Virus M2 Protein
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Product promise

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