Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free

6 product images

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  Flow Cytometry (Intracellular) - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (ab313890)

  This data was developed using Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell) transfected with an Influenza A virus M2 expression vector containing a myc tag cells labelling Influenza A Virus M2 Protein with Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) . A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.

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  Indirect ELISA - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (ab313890)

  This data was developed using Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889, the same antibody clone in a different buffer formulation.
  Indirect ELISA analysis of abab313889 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution. Antigen: Influenza A Virus M2 Protein peptide1, Influenza A Virus M2 Protein peptide2. Antigen concentration: 1000 ng/ml

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  Western blot - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (ab313890)

  This data was developed using Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  
  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
  In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution
  Exposure time: 59 seconds

  All lanes: Western blot - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] (Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with an Influenza A virus M2 expression vector containing a His-tag, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 20 kDa

  Exposure time: 59s

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  Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (ab313890)

  This data was developed using Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889, the same antibody clone in a different buffer formulation.
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A Virus M2 Protein with Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889 at 1/100 (5.26 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in 293T cells transfected with an Influenza A virus M2 expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain at 1/100 (5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (ab313890)

  This data was developed using Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Influenza A Virus M2 Protein with Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889 at 1/4000 (0.132 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cerebrum.The section was incubated with Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] - BSA and Azide free (ab313890)

  This data was developed using Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Influenza A Virus M2 Protein with Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889 at 1/4000 (0.132 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive on (A) HEK-293T transfected with an Influenza A virus M2 expression vector containing a myc tag; no staining on (B) HEK-293T transfected with an empty vector. The section was incubated with Anti-Influenza A Virus M2 Protein antibody [EPR28252-11] ab313889 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins