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Rabbit Recombinant Monoclonal NS1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A, Recombinant fragment - Influenza A samples.

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Images

Flow Cytometry (Intracellular) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (AB313342), expandable thumbnail
  • Indirect ELISA - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (AB313342), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (AB313342), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (AB313342), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (AB313342), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)I-ELISA
Influenza A
Tested
Tested
Tested
Tested
Expected
Recombinant fragment - Influenza A
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Transfected cell line - Influenza A
Not recommended
Tested
Tested
Tested
Not recommended
Transfected cell lysate - Influenza A
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Influenza A, Transfected cell lysate - Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Influenza A, Recombinant fragment - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Influenza A
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell line - Influenza A
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Influenza A, Recombinant fragment - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Influenza A, Transfected cell line - Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Influenza A, Recombinant fragment - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Influenza A, Transfected cell line - Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Influenza A, Recombinant fragment - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Influenza A
Dilution info
-
Notes

-

Expected
Expected

Species
Influenza A
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Influenza A, Transfected cell line - Influenza A
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism. Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated RIGI ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors IRF3 and IRF7. Also binds poly(A) and U6 snRNA. Inhibits the integrated stress response (ISR) in the infected cell by blocking dsRNA binding by EIF2AK2/PKR and further phosphorylation of EIF2S1/EIF-2ALPHA (PubMed:33766561). Stress granule formation is thus inhibited, which allows protein synthesis and viral replication (PubMed:33766561).

Alternative names

Non-structural protein 1, NS1, NS1A, NS

Recommended products

Rabbit Recombinant Monoclonal NS1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A, Recombinant fragment - Influenza A samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28247-51
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Influenza A Nonstructural Protein 1 often referred to as NS1 is an important component of the influenza virus. This protein has an approximate molecular mass of 26 kDa. NS1 is expressed within infected host cells and functions predominantly in the cytoplasm and nucleus. Mechanically NS1 is known for its ability to bind double-stranded RNA effectively modulating host immune responses by interfering with the host's antiviral mechanisms.

Biological function summary

NS1 impacts several critical processes by inhibiting the host cell's immune response. It is not part of a larger protein complex yet it interacts with other cellular factors to suppress the production of interferon an important molecule in antiviral defense. Through its multifunctional activities including binding to cellular proteins and RNA NS1 alters normal host cell processes to benefit viral replication and persistence.

Pathways

NS1 strategically manipulates the host's cellular pathways to thwart the immune response. Two major pathways affected include the interferon signaling pathway where NS1 prevents the activation and transcription of interferon and the apoptosis pathway where it limits cell death aiding in viral replication. NS1 also interacts with proteins like RIG-I which are important in sensing viral RNA and initiating immune responses.

Associated diseases and disorders

NS1 plays a significant role in the pathogenesis of influenza infections such as seasonal flu and pandemic influenza. Its ability to suppress the host's immune response can exacerbate these conditions leading to increased disease severity. Additionally NS1's interactions with other viral proteins like HA and NA contribute to the virus's overall pathogenicity and adaptation to host defenses. Understanding NS1's functionalities can aid in developing therapeutic strategies and vaccines against influenza-related illnesses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry (Intracellular) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342)

    This data was developed using Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a Influenza A virus NS1 protein expression vector containing a myc tag (Middle) / 293T cells transfected with a empty expression vector containing a myc tag (Right) cells labelling Influenza A virus NS1 protein with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 at 1/500 dilution (0.1 ug)/ Middle and Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Indirect ELISA - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342), expandable thumbnail

    Indirect ELISA - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342)

    This data was developed using Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341, the same antibody clone in a different buffer formulation.
    Indirect ELISA analysis of Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
    Antigen: Influenza A virus NS1 protein.
    Antigen concentration: 1000 ng/ml

  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342)

    This data was developed using Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A virus NS1 protein with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in 293T cells transfected with an Influenza A virus NS1 protein expression vector containing a myc tag.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342)

    This data was developed using Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Influenza A virus NS1 protein with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 at 1/2000 (0.25 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat liver. The section was incubated with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342)

    This data was developed using Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Influenza A virus NS1 protein with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 at 1/2000 (0.25 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse liver. The section was incubated with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342), expandable thumbnail

    Western blot - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342)

    This data was developed using Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 3 seconds

    All lanes: Western blot - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] (Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney) transfected with an empty vector (vector control) containi a myc-His-tag® whole cell lysate at 4 µg

    Lane 2: HEK-293 transfected with a Influenza A virus NS1 protein expression vector containi a myc-His-tag® whole cell lysate at 4 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 26 kDa

    Exposure time: 3s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342)

    This data was developed using Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Influenza A virus NS1 protein with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 at 1/2000 (0.25 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human kidney. The section was incubated with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus NS1 protein antibody [EPR28247-51] - BSA and Azide free (ab313342)

    This data was developed using Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T c tissue labeling Influenza A virus NS1 protein with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 at 1/2000 (0.25 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on (A) HEK-293T transfected with a Influenza A virus NS1 protein expression vector and no staining on (B) HEK-293T transfected with empty vector. The section was incubated with Anti-Influenza A virus NS1 protein antibody [EPR28247-51] ab313341 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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