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Anti-Influenza A Virus Nucleoprotein antibody C43 is a mouse monoclonal antibody that is used to detect Influenza A Virus Nucleoprotein in Western blot, ICC/IF, Flow cytometry, ELISA.

- Cited in over 40 publications
- Antibody clone C43 is cited in over 50 publications

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (AB128193), expandable thumbnail
  • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (AB128193), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (AB128193), expandable thumbnail
  • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (AB128193), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Virus preparation containing NP protein. The exact immunogen used to generate this antibody is proprietary information. Database link P69291

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytELISAWBICC/IF
Influenza A
Expected
Expected
Predicted
Predicted
Transfected cell line - Influenza A
Not recommended
Not recommended
Tested
Tested

Expected
Expected

Species
Influenza A
Dilution info
-
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Transfected cell line - Influenza A
Dilution info
-
Notes

-

Expected
Expected

Species
Influenza A
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Influenza A
Dilution info
1/300.00000 - 1/1000.00000
Notes

-

Predicted
Predicted

Species
Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Influenza A
Dilution info
1/200
Notes

-

Predicted
Predicted

Species
Influenza A
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Additional Targets

Influenza A Virus Nucleoprotein

Alternative names

Nucleoprotein, Nucleocapsid protein, Protein N, NP

Recommended products

Anti-Influenza A Virus Nucleoprotein antibody C43 is a mouse monoclonal antibody that is used to detect Influenza A Virus Nucleoprotein in Western blot, ICC/IF, Flow cytometry, ELISA.

- Cited in over 40 publications
- Antibody clone C43 is cited in over 50 publications

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Virus preparation containing NP protein. The exact immunogen used to generate this antibody is proprietary information. Database link P69291
Clone number
C43
Purification technique
Proprietary technique
Specificity

Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34).

No cross reactivity with influenza B viruses.

Concentration
Loading...
Purification notes

Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt, ICC/IF, WB in influenza a samples.
Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) specifically detects Influenza A Virus Nucleoprotein (UniProt ID: P69291; Molecular weight: 56kDa) and is sold in 100 µg selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) has high sensitivity and specificity.
Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) has been cited over 42 times in peer reviewed journals and is trusted by the scientific community.


Related Products

Antibody clone C43 is also available pre-conjugated to a variety of labels for your convenience - HRP (HRP Anti-Influenza A Virus Nucleoprotein antibody [C43] ab176837).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Influenza A Virus Nucleoprotein also known as NP or nucleoprotein influenza A plays a significant mechanical role in the virus life cycle. It is an essential structural component that encapsidates viral RNA forming ribonucleoprotein complexes important for replication and transcription. Influenza A nucleoprotein has a molecular weight of approximately 56 kDa and is expressed in infected host cells. Within the influenza virion the nucleoprotein interacts with the viral RNA and polymerase complex ensuring the integrity and functionality of the viral genome.

Biological function summary

The influenza A nucleoprotein is involved in regulating viral RNA synthesis and acts as a platform for the assembly of viral components. It does not directly form stable complexes with host proteins but it is central to the ribonucleoprotein complexes that are vital for the virus life cycle. This nucleoprotein assists in the virus's ability to hijack the host cellular machinery favoring its replication and infection processes. While the nucleoprotein itself does not form direct complexes with host proteins its interactions are significant for facilitating various stages of the viral life cycle.

Pathways

The influenza A nucleoprotein operates within the viral replication and transcription pathways. It associates with proteins such as PB1 PB2 and PA of the polymerase complex which is important for viral RNA synthesis. The nucleoprotein ensures the proper packaging of the viral genome to be recognized by the polymerase complex playing a pivotal role in the viral replication process. Through these pathways it indirectly impacts the host immune response as the virus must evade host defenses to ensure replication success.

Associated diseases and disorders

The nucleoprotein influenza A is primarily linked to influenza infections a major respiratory illness. Influenza can manifest in a mild to severe form occasionally leading to pandemics. The nucleoprotein is an attractive target for antiviral drug development given its essential role in the viral life cycle. Moreover studies have shown that variations and mutations in the nucleoprotein can affect viral virulence and the outcome of infection. By understanding its mechanisms and interactions with other viral proteins like HA and NA researchers can better develop strategies to combat influenza outbreaks.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)

    Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from
    human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were
    taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline
    (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further
    reaction with an green flurescent secondary antibody.

  • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193), expandable thumbnail

    Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)

    All lanes: Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution

    All lanes: MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34

    Secondary

    All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (Rabbit Anti-Mouse IgG H&L (HRP) ab97046) at 1/10000 dilution

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)

    Immunofluorescence assay of 293T cells
    expressing HA or NP of pandemic (H1N1) 2009
    influenza A virus (A/Suita/1/2009).
    Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells
    while a proprietary mouse anti-HA
    monoclonal antibody specifically recognized HA.

  • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193), expandable thumbnail

    Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)

    Tubulin was used as loading control.

    All lanes: Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution

    Lanes 1, 6 and 11: MDCK cells mock infected

    Lane 2: MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection

    Lane 3: MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection

    Lane 4: MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection

    Lane 5: MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection

    Lane 7: MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection

    Lane 8: MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection

    Lane 9: MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection

    Lane 10: MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection

    Lane 12: MDCK cells infected H5N1 (A/duck/HK/342/78)collected 3 hrs post-infection

    Lane 13: MDCK cells infected with H5N1 (A/duck/HK/342/78) collected 9 hrs post-infection

    Lane 14: MDCK cells infected with H5N1 (A/duck/HK/342/78) collected 24 hrs post-infection

    Lane 15: MDCK cells infected with H5N1 (A/duck/HK/342/78) collected 48 hrs post-infection

    Predicted band size: 56 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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