Anti-Influenza A Virus Nucleoprotein antibody [C43]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (AB128193)

Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an green flurescent secondary antibody.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (AB128193)

Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells while a proprietary mouse anti-HA monoclonal antibody specifically recognized HA.

• WB

Unknown

Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (AB128193)

Tubulin was used as loading control.

All lanes:

Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution

Lanes 1, 6 and 11:

MDCK cells mock infected

Lane 2:

MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection

Lane 3:

MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection

Lane 4:

MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection

Lane 5:

MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection

Lane 7:

MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection

Lane 8:

MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection

Lane 9:

MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection

Lane 10:

MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection

Lane 12:

MDCK cells infected H5N1 (A/duck/HK/342/78)collected 3 hrs post-infection

Lane 13:

MDCK cells infected with H5N1 (A/duck/HK/342/78) collected 9 hrs post-infection

Lane 14:

MDCK cells infected with H5N1 (A/duck/HK/342/78) collected 24 hrs post-infection

Lane 15:

MDCK cells infected with H5N1 (A/duck/HK/342/78) collected 48 hrs post-infection

Predicted band size: 56 kDa

false

• WB

Supplier Data

Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (AB128193)

All lanes:

Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution

All lanes:

MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/rabbit-mouse-igg-h-l-hrp-ab97046'>ab97046</a>) at 1/10000 dilution

Predicted band size: 56 kDa

false