Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A, Recombinant fragment - Influenza A samples.
Images
![Flow Cytometry (Intracellular) - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-nucleoprotein-antibody-epr28253-17-bsa-and-azide-free-ab313344--flow-cytometry-intracellular-img417514.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344)")
![Indirect ELISA - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-nucleoprotein-antibody-epr28253-17-bsa-and-azide-free-ab313344--indirect-elisa-img417518.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344)")
![Western blot - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-nucleoprotein-antibody-epr28253-17-bsa-and-azide-free-ab313344--western-blot-img417496.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344)")
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-nucleoprotein-antibody-epr28253-17-bsa-and-azide-free-ab313344--immunocytochemistry-immunofluorescence-img417512.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | I-ELISA | IHC-P | |
|---|---|---|---|---|---|
| Influenza A | Tested | Tested | Tested | Expected | Not recommended |
| Recombinant fragment - Influenza A | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Transfected cell line - Influenza A | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Transfected cell lysate - Influenza A | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A, Transfected cell lysate - Influenza A | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Influenza A, Recombinant fragment - Influenza A | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A, Transfected cell line - Influenza A | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Influenza A, Recombinant fragment - Influenza A | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A, Transfected cell line - Influenza A | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Influenza A, Recombinant fragment - Influenza A | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Influenza A | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Influenza A, Transfected cell line - Influenza A | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A, Recombinant fragment - Influenza A | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
Alternative names
Nucleoprotein, Nucleocapsid protein, Protein N, NP
Recommended products
Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), I-ELISA and reacts with Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A, Recombinant fragment - Influenza A samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28253-17
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Influenza A Virus Nucleoprotein also known as NP or nucleoprotein influenza A plays a significant mechanical role in the virus life cycle. It is an essential structural component that encapsidates viral RNA forming ribonucleoprotein complexes important for replication and transcription. Influenza A nucleoprotein has a molecular weight of approximately 56 kDa and is expressed in infected host cells. Within the influenza virion the nucleoprotein interacts with the viral RNA and polymerase complex ensuring the integrity and functionality of the viral genome.
- Biological function summary
The influenza A nucleoprotein is involved in regulating viral RNA synthesis and acts as a platform for the assembly of viral components. It does not directly form stable complexes with host proteins but it is central to the ribonucleoprotein complexes that are vital for the virus life cycle. This nucleoprotein assists in the virus's ability to hijack the host cellular machinery favoring its replication and infection processes. While the nucleoprotein itself does not form direct complexes with host proteins its interactions are significant for facilitating various stages of the viral life cycle.
- Pathways
The influenza A nucleoprotein operates within the viral replication and transcription pathways. It associates with proteins such as PB1 PB2 and PA of the polymerase complex which is important for viral RNA synthesis. The nucleoprotein ensures the proper packaging of the viral genome to be recognized by the polymerase complex playing a pivotal role in the viral replication process. Through these pathways it indirectly impacts the host immune response as the virus must evade host defenses to ensure replication success.
- Associated diseases and disorders
The nucleoprotein influenza A is primarily linked to influenza infections a major respiratory illness. Influenza can manifest in a mild to severe form occasionally leading to pandemics. The nucleoprotein is an attractive target for antiviral drug development given its essential role in the viral life cycle. Moreover studies have shown that variations and mutations in the nucleoprotein can affect viral virulence and the outcome of infection. By understanding its mechanisms and interactions with other viral proteins like HA and NA researchers can better develop strategies to combat influenza outbreaks.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry (Intracellular) - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-nucleoprotein-antibody-epr28253-17-bsa-and-azide-free-ab313344--flow-cytometry-intracellular-img417514.jpg "Flow Cytometry (Intracellular) - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344)")
Flow Cytometry (Intracellular) - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344)
This data was developed using Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] ab313343, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a Influenza A Virus Nucleoprotein expression vector containing a myc tag (Middle) / 293T cells transfected with a empty expression vector containing a myc tag (Right) cells labelling Influenza A Virus Nucleoprotein with Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] ab313343 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![Indirect ELISA - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-nucleoprotein-antibody-epr28253-17-bsa-and-azide-free-ab313344--indirect-elisa-img417518.jpg "Indirect ELISA - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344)")
Indirect ELISA - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344)
This data was developed using Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] ab313343, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] ab313343 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Influenza A Virus Nucleoprotein.
Antigen concentration: 1000 ng/ml![Western blot - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-nucleoprotein-antibody-epr28253-17-bsa-and-azide-free-ab313344--western-blot-img417496.jpg "Western blot - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344)")
Western blot - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344)
This data was developed using Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] ab313343, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 secondsAll lanes: Western blot - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] (Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] ab313343) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containi a His tag whole cell lysate at 20 µg
Lane 2: 293T transfected with an Influenza A Virus Nucleoprotein expression vector containi a His tag whole cell lysate at 20 µg
Lane 3: 293T transfected with an Influenza B Virus Nucleoprotein expression vector containi a His tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 56 kDa
Exposure time: 15s
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344), expandable thumbnail](https://content.abcam.com/products/images/influenza-a-virus-nucleoprotein-antibody-epr28253-17-bsa-and-azide-free-ab313344--immunocytochemistry-immunofluorescence-img417512.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344)")
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (ab313344)
This data was developed using Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] ab313343, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling Influenza A Virus Nucleoprotein with Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] ab313343 at 1/250 (2.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing positive staining in 293T cells transfected with an Influenza A Virus Nucleoprotein expression vector containing a myc tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
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