Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344)

This data was developed using ab313343, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling Influenza A Virus Nucleoprotein with ab313343 at 1/250 (2.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing positive staining in 293T cells transfected with an Influenza A Virus Nucleoprotein expression vector containing a myc tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344)

This data was developed using ab313343, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a Influenza A Virus Nucleoprotein expression vector containing a myc tag (Middle) / 293T cells transfected with a empty expression vector containing a myc tag (Right) cells labelling Influenza A Virus Nucleoprotein with ab313343 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• I-ELISA

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Indirect ELISA - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344)

This data was developed using ab313343, the same antibody clone in a different buffer formulation. Indirect ELISA analysis of ab313343 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution. Antigen : Influenza A Virus Nucleoprotein. Antigen concentration : 1000 ng/ml

• WB

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Western blot - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] - BSA and Azide free (AB313344)

This data was developed using ab313343, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-Influenza A Virus Nucleoprotein antibody [EPR28253-17] (<a href='/en-us/products/primary-antibodies/influenza-a-virus-nucleoprotein-antibody-epr28253-17-ab313343'>ab313343</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His tag whole cell lysate at 20 µg

Lane 2:

293T transfected with an Influenza A Virus Nucleoprotein expression vector containi a His tag whole cell lysate at 20 µg

Lane 3:

293T transfected with an Influenza B Virus Nucleoprotein expression vector containi a His tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 56 kDa

false

Exposure time: 15s