Skip to main content

Rabbit Recombinant Monoclonal Polymerase acidic protein antibody. Suitable for I-ELISA, WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Recombinant fragment - Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A samples.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (AB313745), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (AB313745), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (AB313745), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (AB313745), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (AB313745), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAWBIHC-PICC/IFFlow Cyt (Intra)IP
Influenza A
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Recombinant fragment - Influenza A
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Influenza A
Not recommended
Not recommended
Tested
Tested
Tested
Not recommended
Transfected cell lysate - Influenza A
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species
Recombinant fragment - Influenza A
Dilution info
1000 ng/mL
Notes

-

Predicted
Predicted

Species
Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Influenza A, Transfected cell line - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Influenza A
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Influenza A, Transfected cell line - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Influenza A
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Influenza A, Transfected cell lysate - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Influenza A
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Influenza A, Transfected cell lysate - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Influenza A
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Influenza A, Transfected cell lysate - Influenza A
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Influenza A
Dilution info
1/30
Notes

-

Predicted
Predicted

Species
Influenza A
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Influenza A, Transfected cell line - Influenza A
Dilution info
-
Notes

-

Target data

Function

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

Alternative names

Polymerase acidic protein, RNA-directed RNA polymerase subunit P2, PA

Recommended products

Rabbit Recombinant Monoclonal Polymerase acidic protein antibody. Suitable for I-ELISA, WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Recombinant fragment - Influenza A, Transfected cell lysate - Influenza A, Transfected cell line - Influenza A samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28250-39
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Influenza A virus PA protein also known simply as PA protein plays an important mechanical role in the viral replication process. It acts as a component of the RNA polymerase complex within the Influenza A virus. This protein has a molecular mass of approximately 85 kDa. PA is expressed in infected host cells and contributes significantly to viral RNA synthesis by possessing endonuclease activity which is important for the cleavage of host mRNA during viral transcription.

Biological function summary

This protein is part of the viral RNA polymerase complex along with PB1 and PB2 proteins. This complex functions as an essential machinery for the transcription and replication of the viral RNA genome enabling the virus to use host cellular machinery for its proliferation. PA protein interacts specifically with the PB1 protein assisting in the polymerase assembly and activity which marks it as an important element in the life cycle of the virus.

Pathways

The PA protein is involved primarily in the influenza viral RNA synthesis pathway. The polymerase complex which includes PA drives this pathway by transcribing viral RNA into mRNA and replicating the viral genome. The PA protein also interacts with the host's nuclear pathways as influenza replication occurs in the host cell nucleus. Its interaction with PB1 and PB2 proteins is central to these pathways facilitating the initiation and elongation phases of RNA synthesis.

Associated diseases and disorders

The PA protein relates directly to influenza infections in humans which can manifest as seasonal flu outbreaks or pandemic events. Its role in the replication of Influenza A makes it a potential target for antiviral drug development. Compounds that inhibit the endonuclease activity of PA protein aim to impair viral growth and spread. The PA protein's interaction with other viral proteins like PB1 highlights its involvement in the pathogenesis of influenza and highlights its importance in the disease life cycle.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A Virus PA protein with ab313745 at 1/500 (1.016 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining in 293T cells transfected with a Influenza A Virus PA protein expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Influenza A Virus PA protein with ab313745 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat kidney. The section was incubated with ab313745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Influenza A Virus PA protein with ab313745 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human kidney. The section was incubated with ab313745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Influenza A Virus PA protein with ab313745 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse kidney. The section was incubated with ab313745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T tissue labeling Influenza A Virus PA protein with ab313745 at 1/2000 (0.254 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on (A) HEK-293T transfected with a Influenza A Virus PA protein expression vector and no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab313745 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Immunoprecipitation - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Influenza A Virus PA protein was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with a Influenza A Virus PA protein expression vector containing a His-tag whole cell lysate with ab313745 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313745 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a Influenza A Virus PA protein expression vector containing a His-tag whole cell lysate
    Lane 2: 293T (human embryonic kidney epithelial cell) cells transfected with a Influenza A Virus PA protein expression vector containing a His-tag whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313745 in 293T whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds

    All lanes: Immunoprecipitation - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745) at 1/30 dilution

    All lanes: 293T (human embryonic kidney epithelial cell) cells transfected with a Influenza A Virus PA protein expression vector containing a His-tag whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 5s

  • Western blot - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Western blot - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The lower MW band at approximately 65kDa is expected to be a degradation product.
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 5.5 seconds

    All lanes: Western blot - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Influenza A Virus PA protein expression vector containing a His-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 83 kDa

    Exposure time: 5.5s

  • Flow Cytometry (Intracellular) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T cells (human embryonic kidney epithelial cell) transfected with a Influenza A Virus PA protein expression vector containing a Myc-His tag cells labelling Influenza A Virus PA protein with ab313745 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Indirect ELISA - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745), expandable thumbnail

    Indirect ELISA - Anti-Influenza A Virus PA protein antibody [EPR28250-39] (ab313745)

    Indirect ELISA analysis of abab313745 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
    Antigen: Influenza A Virus PA protein.
    Antigen concentration: 1000 ng/ml

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com