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Rabbit Recombinant Monoclonal Influenza A virus PA protein (A/WSN/1933(H1N1)) antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line, Purified native protein samples.

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Images

Western blot - Anti-Influenza A virus PA protein antibody [HL1388] (AB308362), expandable thumbnail
  • Western blot - Anti-Influenza A virus PA protein antibody [HL1388] (AB308362), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus PA protein antibody [HL1388] (AB308362), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Purified native protein
Not recommended
Tested
Transfected cell line
Tested
Tested

Tested
Tested

Species
Transfected cell line
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Purified native protein
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/500.00000 - 1/1000.00000
Notes

-

Species
Purified native protein
Dilution info
1/500.00000 - 1/1000.00000
Notes

-

Target data

Recommended products

Rabbit Recombinant Monoclonal Influenza A virus PA protein (A/WSN/1933(H1N1)) antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line, Purified native protein samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Carrier free
Yes
Clone number
HL1388
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Influenza A virus PA protein antibody [HL1388] (ab308362), expandable thumbnail

    Western blot - Anti-Influenza A virus PA protein antibody [HL1388] (ab308362)

    Influenza A viral lysate and Influenza B viral lysate (0.5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody [HL1388] (ab308362) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Influenza A virus PA protein antibody [HL1388] (ab308362) at 1/1000 dilution

    Lane 1: A/Brisbane/59/07 (H1N1) viral lysate at 0.5 µg

    Lane 2: A/NewCal/20/99 (H1N1) viral lysate at 0.5 µg

    Lane 3: A/PuertoRico/8/34 (H1N1) viral lysate at 0.5 µg

    Lane 4: A/Taiwan/42/06 (H1N1) viral lysate at 0.5 µg

    Lane 5: A/California/07/09 (H1N1) viral lysate at 0.5 µg

    Lane 6: A/Mexico/4108/09 (H1N1) viral lysate at 0.5 µg

    Lane 7: A/NewYork/01/09 (H1N1) viral lysate at 0.5 µg

    Lane 8: A/NewYork/02/09 (H1N1) viral lysate at 0.5 µg

    Lane 9: A/NewYork/03/09 (H1N1) viral lysate at 0.5 µg

    Lane 10: A/Canada/6294/09 (H1N1) viral lysate at 0.5 µg

    Lane 11: A/Singapore/63/04 (H1N1) viral lysate at 0.5 µg

    Lane 12: A/Brisbane/10/07 (H3N2) viral lysate at 0.5 µg

    Lane 13: A/Perth/16/09 (H3N2) viral lysate at 0.5 µg

    Lane 14: A/Texas/50/12 (H3N2) viral lysate at 0.5 µg

    Lane 15: A/Wisconsin/67/05 (H3N2) viral lysate at 0.5 µg

    Lane 16: A/HongKong/8/68 (H3N2) viral lysate at 0.5 µg

    Lane 17: B/Florida/02/06 viral lysate at 0.5 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-Influenza A virus PA protein antibody [HL1388] (ab308362), expandable thumbnail

    Western blot - Anti-Influenza A virus PA protein antibody [HL1388] (ab308362)

    Non-infected (–) and infected (+) H1299 whole cell extracts (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody [HL1388] (ab308362) diluted at 1/500. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Influenza A virus PA protein antibody [HL1388] (ab308362) at 1/500 dilution

    Lane 1: Non-infected (–) H1299 whole cell lysate at 1 µg

    Lane 2: Infected (+) H1299 whole cell lysate at 1 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus PA protein antibody [HL1388] (ab308362), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus PA protein antibody [HL1388] (ab308362)

    For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Influenza A virus PA protein using ab308362 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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