Anti-Influenza A virus PB1 antibody [EPR28248-26]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus PB1 antibody [EPR28248-26] (AB313887)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Influenza A virus PB1 protein with ab313887 at 1/1000 (0.5 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control : No staining on human endometrium. The section was incubated with ab313887 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus PB1 antibody [EPR28248-26] (AB313887)

Immunohistochemical analysis of paraffin-embedded (A) 293T (human epithelial cell line from embryonic kidney) transfected with an Influenza A virus PB1 expression vector containing a Myc-his tag and (B) 293T transfected with empty vector containing a Myc-his tag with ab313887 at 1/1000 (0.5 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on (A) 293T (human epithelial cell line from embryonic kidney) transfected with an Influenza A virus PB1 expression vector containing a Myc-his tag, no staining on (B) 293T transfected with empty vector containing a Myc-his tag. The section was incubated with ab313887 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Influenza A virus PB1 antibody [EPR28248-26] (AB313887)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell) transfected with a Influenza A virus PB1 protein expression vector containing a myc tag cells labelling Influenza A virus PB1 protein with ab313887 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus PB1 antibody [EPR28248-26] (AB313887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A virus PB1 protein with ab313887 at 1/1000 (0.5 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing positive staining staining in 293T cells transfected with a Influenza A virus PB1 protein expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8) Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 (0.38 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• WB

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Western blot - Anti-Influenza A virus PB1 antibody [EPR28248-26] (AB313887)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution Exposure time : 8 seconds

All lanes:

Western blot - Anti-Influenza A virus PB1 antibody [EPR28248-26] (ab313887) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 7 µg

Lane 2:

293T cells transfected with an Influenza A virus PB1 expression vector containing a myc-His-tag® whole cell lysate at 7 µg

Secondary

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Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 87 kDa

false

Exposure time: 8s

• I-ELISA

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Indirect ELISA - Anti-Influenza A virus PB1 antibody [EPR28248-26] (AB313887)

Indirect ELISA analysis of ab313887 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution. Antigen : Influenza A virus PB1 protein. Antigen concentration : 1000 ng/ml.