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AB308400

Anti-Influenza A virus PB1 protein antibody [HL1715]

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Rabbit Recombinant Monoclonal Influenza A virus PB1 protein antibody. Carrier free. Suitable for WB, IHC-P and reacts with Purified native protein - Influenza A, Transfected cell line - Influenza A samples.

View Alternative Names

PB1-F2 protein, Protein PB1-F2

3 Images
Western blot - Anti-Influenza A virus PB1 protein antibody [HL1715] (AB308400)
  • WB

Supplier Data

Western blot - Anti-Influenza A virus PB1 protein antibody [HL1715] (AB308400)

Non-transfected (–) and transfected (+) HEK293T whole cell lysates (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody [HL1715] (ab308400) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Influenza A virus PB1 protein antibody [HL1715] (ab308400) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK293T whole cell lysates at 30 µg

Lane 2:

Transfected (+) HEK293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus PB1 protein antibody [HL1715] (AB308400)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus PB1 protein antibody [HL1715] (AB308400)

Paraffin-embedded mock and Influenza A virus PB1 protein transfected 293T tissue stained for Influenza A virus PB1 protein with ab308400 at a 1/1000 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-Influenza A virus PB1 protein antibody [HL1715] (AB308400)
  • WB

Supplier Data

Western blot - Anti-Influenza A virus PB1 protein antibody [HL1715] (AB308400)

All lanes:

Western blot - Anti-Influenza A virus PB1 protein antibody [HL1715] (ab308400) at 1/5000 dilution

All lanes:

Influenza A (H1N1) viral lysate at 0.5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1715

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A

Applications

WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Purified native protein - Influenza A": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell line - Influenza A": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Cell pellet</p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Influenza A PB1 protein also known as polymerase basic protein 1 forms a core part of the viral polymerase complex. It is a large protein with a molecular weight of approximately 85 kDa. PB1 expresses predominantly in the host cell cytoplasm and nucleus following an Influenza A virus infection. This protein orchestrates the replication and transcription of the viral RNA genome by taking part in the assembly of the viral RNA-dependent RNA polymerase. PB1 interacts with other subunits including PB2 and PA to form this essential polymerase complex.
Biological function summary

This viral protein facilitates the copying of viral RNA into progeny genomes and viral mRNA transcripts for protein synthesis. The PB1 component of the polymerase complex incorporates both the template-moving catalytic activities and binding sites. The functional partnerships between PB1 PB2 and PA bring the necessary enzymatic activities for efficient viral gene expression. Its role in the context of the polymerase complex makes it an important factor for the continuation of the viral life cycle ensuring efficient replication of the virus within the host.

Pathways

PB1 is vital to the viral replication pathway in that it interacts with other polymerase basic proteins like PB2 and related accessory proteins. This pathway oversees the synthesis of viral RNA in both transcriptional and replicative formats. It is closely related to the nuclear export by functioning with the PA and PB2 in the transport of new virus genomes and associated proteins to the host cell cytoplasm eventually leading to the assembly and release of infectious virions.

Influenza A PB1 contributes critically to the pathogenicity and spread of influenza infections in humans and animals. It is notably associated with seasonal flu outbreaks and pandemics. The PB1 protein's interactions with other surface proteins like HA (hemagglutinin) and NA (neuraminidase) influence virulence and transmissibility. Mutations in PB1 can alter the severity and outcome of flu infections making it a target for antiviral drug development and vaccine design aimed at mitigating the impact of influenza-induced respiratory illnesses and complications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Influenza A virus PB1 protein
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com