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AB308216

Anti-Influenza A virus PB2 protein antibody [HL1992]

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Rabbit Recombinant Monoclonal Influenza A virus PB2 protein antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Influenza A virus (A/Puerto Rico/8/1934(H1N1)), Influenza A samples.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus PB2 protein antibody [HL1992] (AB308216)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus PB2 protein antibody [HL1992] (AB308216)

Immunofluorescent analysis of Influenza A and B virus infected cells labelling Influenza A virus PB2 protein with ab308216 at 1/1000 dilution.

Blue : Fluoroshield with DAPI.

Western blot - Anti-Influenza A virus PB2 protein antibody [HL1992] (AB308216)
  • WB

Supplier Data

Western blot - Anti-Influenza A virus PB2 protein antibody [HL1992] (AB308216)

Influenza A viral lysate was separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB2 protein antibody [HL1992] (ab308216) diluted at 1 : 10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Influenza A virus PB2 protein antibody [HL1992] (ab308216) at 1/10000 dilution

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1992

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza A

Applications

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza A": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Influenza A virus (A/Puerto Rico/8/1934(H1N1))": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A Polymerase basic protein 2 also known as PB2 protein plays an important mechanical role in the viral RNA polymerase complex which is necessary for viral replication. This protein has a molecular mass of approximately 87 kDa. PB2 is expressed within infected host cells and is a component of the influenza A virus polymerase complex along with PA and PB1 proteins. The PB2 target is essential for the transcription and replication phase in the viral lifecycle allowing the virus to hijack the host cell machinery for viral propagation.
Biological function summary

PB2 protein is part of the heterotrimeric RNA polymerase complex which is critical for the influenza virus's ability to replicate. The polymerase complex includes PA PB1 and PB2 proteins where PB2 protein plays a role in cap-snatching a mechanism to steal host mRNA caps for viral mRNA synthesis. This enables the influenza virus to synthesize its own proteins in the host cytoplasm facilitating efficient viral replication and protein expression.

Pathways

The PB2 protein functions prominently within the viral replication cycle of the influenza A virus. It is intricately linked with the host's mRNA processing pathways as it engages in the cap-snatching mechanism necessary for viral mRNA transcription. PB2 protein works closely with PB1 in the replication pathway where PB1 acts as the RNA-dependent RNA polymerase in conjunction with activities of PB2.

PB2 protein plays a significant role in the pathogenicity of influenza A virus infections in humans and animals. Its interactions are pivotal for the adaptation and virulence of the influenza virus leading to significant morbidity during outbreaks. Mutations in the PB2 gene can relate to species adaptation and increased virulence and the protein's function is important for the virus to overcome host defense mechanisms frequently. PB2 interacts with host proteins to moderate host immune responses making it a target for antiviral drug development and treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Influenza A virus PB2 protein
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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