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AB317162

Anti-Influenza B virus Hemagglutinin (HA) antibody [HL2208]

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Rabbit Monoclonal antibody. Suitable for WB and reacts with Influenza B, Transfected cell lysate - Influenza B samples.
2 Images
Western blot - Anti-Influenza B virus Hemagglutinin (HA) antibody [HL2208] (AB317162)
  • WB

Supplier Data

Western blot - Anti-Influenza B virus Hemagglutinin (HA) antibody [HL2208] (AB317162)

Non-infected (–) and infected (+) MDCK whole cell extracts (1 ug MDCK | B/Taiwan/70555/05 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317162 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Influenza B virus Hemagglutinin (HA) antibody [HL2208] (ab317162) at 1/1000 dilution

Lane 1:

Non-infected (–) MDCK whole cell extracts at 1 µg

Lane 2:

Influenza B virus infected (+) MDCK whole cell extracts at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Influenza B virus Hemagglutinin (HA) antibody [HL2208] (AB317162)
  • WB

Supplier Data

Western blot - Anti-Influenza B virus Hemagglutinin (HA) antibody [HL2208] (AB317162)

Influenza A (H1N1) and Influenza B viral lysate were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317162 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Influenza B virus Hemagglutinin (HA) antibody [HL2208] (ab317162) at 1/1000 dilution

Lane 1:

Influenza A (H1N1) viral lysate

Lane 2:

Influenza B viral lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2208

Isotype

IgG

Carrier free

No

Reacts with

Influenza B

Applications

WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza B": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Influenza B": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza B virus hemagglutinin also known as HA is a glycoprotein expressed on the surface of the Influenza B virus. It has a molecular mass of approximately 76 kDa. HA is responsible for binding the virus to sialic acid receptors on host cells facilitating viral entry. This binding occurs via the HA1 subunit while the HA2 subunit enables fusion between viral and endosomal membranes after endocytosis. Hemagglutinin is the first contact point between the virus and host cells playing a central role in the infection process.
Biological function summary

The hemagglutinin protein acts as a part of the viral envelope complex which is paramount for viral infectivity. The interaction between HA and sialic acid receptors determines host specificity often limiting the species and cell types that the virus can infect. HA undergoes post-translational modifications like glycosylation which influences its antigenic properties and immune evasion strategies. The antigenic variability of HA necessitates regular updates to influenza vaccines.

Pathways

Hemagglutinin serves a critical role in the endocytic pathway enabling viral entry into host cells. Once HA induces membrane fusion the viral ribonucleoprotein complex is released into the cytoplasm leading to transport into the nucleus for eventual replication. Hemagglutinin works closely with neuraminidase another surface protein that aids in viral release completing the virus life cycle. The interaction between these two proteins guides the propagation of the virus within the host organism.

Hemagglutinin serves as a central factor in the development of Influenza B virus infection leading to flu epidemics among humans. Variability in the HA protein is largely responsible for seasonal flu outbreaks due to antigenic drift. HA's interaction with the immune system is complex involving the production of neutralizing antibodies that target specific epitopes on HA. Such interactions between HA and the immune system proteins inform vaccine development and therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

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