Anti-Influenza B Virus Neuraminidase [HL1476] antibody (ab313856) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect Influenza B Virus Neuraminidase in Western Blot, IHC-P, ICC/IF. Suitable for Influenza B samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856), expandable thumbnail](https://content.abcam.com/products/images/influenza-b-virus-neuraminidase-antibody-hl1476-bsa-and-azide-free-ab313856--immunocytochemistry-immunofluorescence-img418891.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856), expandable thumbnail](https://content.abcam.com/products/images/influenza-b-virus-neuraminidase-antibody-hl1476-bsa-and-azide-free-ab313856--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418909.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)")
![Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856), expandable thumbnail](https://content.abcam.com/products/images/influenza-b-virus-neuraminidase-antibody-hl1476-bsa-and-azide-free-ab313856--western-blot-img418859.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ICC/IF | IHC-P | WB | |
|---|---|---|---|
| Influenza B | Expected | Expected | Tested |
| Transfected cell line | Tested | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza B | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza B | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza B | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
Alternative names
NA, Neuraminidase, Influenzavirus B neuraminidase
Recommended products
Anti-Influenza B Virus Neuraminidase [HL1476] antibody (ab313856) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect Influenza B Virus Neuraminidase in Western Blot, IHC-P, ICC/IF. Suitable for Influenza B samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1476
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Influenza B samples.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Influenza B Virus Neuraminidase also known as Influenza B NA is an enzyme important for the viral life cycle. This protein has an approximate molecular mass of 60 kDa and is expressed on the surface of the Influenza B virus. Neuraminidase facilitates the release of virions from infected cells by cleaving sialic acid residues aiding viral spread. It acts as a tetramer forming a homotetrameric structure which is essential for its enzymatic activity.
- Biological function summary
The neuraminidase functions as part of the viral replication process. By cleaving sialic acid it prevents viral aggregation at the host cell surface ensuring efficient viral dissemination. This activity complements hemagglutinin another glycoprotein on the viral surface which binds to sialic acid on host cells to initiate infection. Together these proteins orchestrate the infection process marking the structural and functional complexity of the virus.
- Pathways
Neuraminidase plays an integral role in the viral lifecycle pathway of the Influenza B virus. It closely interacts with the HA/NA balance where hemagglutinin (HA) binding necessitates subsequent neuraminidase activity to liberate progeny virions. This interaction is critical for maintaining the balance of viral attachment and release during infection influencing the overall efficiency of viral propagation.
- Associated diseases and disorders
The neuraminidase of the Influenza B virus is a significant target in antiviral drug design particularly in relation to influenza infections. Drugs like oseltamivir target neuraminidase to block its activity reducing viral spread and severity of infection. Furthermore the activity of neuraminidase is associated with resistance mutations influencing the effectiveness of antiviral treatment therefore posing significant challenges in combating influenza outbreaks.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856), expandable thumbnail](https://content.abcam.com/products/images/influenza-b-virus-neuraminidase-antibody-hl1476-bsa-and-azide-free-ab313856--immunocytochemistry-immunofluorescence-img418891.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856)")
Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856)
Immunofluorescent analysis of mock and Influenza B virus Neuraminidase (NA) transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 min labeling Influenza B virus Neuraminidase (NA) with ab313856 at 1/500 dilution (green). Blue: Fluoroshield with DAPI. Scale bar= 10um.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856), expandable thumbnail](https://content.abcam.com/products/images/influenza-b-virus-neuraminidase-antibody-hl1476-bsa-and-azide-free-ab313856--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418909.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856)
Immunohistochemical analysis of paraffin-embedded mock and Influenza B virus Neuraminidase (NA) transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell pellets labeling Influenza B virus Neuraminidase (NA) with ab313856 at 1/1000 dilution. Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.
![Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856), expandable thumbnail](https://content.abcam.com/products/images/influenza-b-virus-neuraminidase-antibody-hl1476-bsa-and-azide-free-ab313856--western-blot-img418859.jpg "Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856)")
Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856)
All lanes: Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856) at 1/5000 dilution
All lanes: Influenza B (Flu B/Florida/07/04) viral lysate at 1 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Downloads
Product protocols
- Visit the general protocols
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