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AB313856

Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free

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Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Influenza B Virus Neuraminidase in Western Blot, IHC-P, ICC/IF.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Recombinant format for unrivalled batch-batch consistency
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)

Immunohistochemical analysis of paraffin-embedded mock and Influenza B virus Neuraminidase (NA) transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell pellets labeling Influenza B virus Neuraminidase (NA) with ab313856 at 1/1000 dilution. Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)

Immunofluorescent analysis of mock and Influenza B virus Neuraminidase (NA) transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells fixed in 4% paraformaldehyde at room temperature for 15 min labeling Influenza B virus Neuraminidase (NA) with ab313856 at 1/500 dilution (green). Blue : Fluoroshield with DAPI. Scale bar= 10um.

Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)
  • WB

Supplier Data

Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (AB313856)

All lanes:

Western blot - Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856) at 1/5000 dilution

All lanes:

Influenza B (Flu B/Florida/07/04) viral lysate at 1 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1476

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza B

Applications

WB, ICC/IF, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza B": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Transfected cell line": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Influenza B Virus Neuraminidase antibody [HL1476] - BSA and Azide free (ab313856) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Influenza B samples.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza B Virus Neuraminidase also known as Influenza B NA is an enzyme important for the viral life cycle. This protein has an approximate molecular mass of 60 kDa and is expressed on the surface of the Influenza B virus. Neuraminidase facilitates the release of virions from infected cells by cleaving sialic acid residues aiding viral spread. It acts as a tetramer forming a homotetrameric structure which is essential for its enzymatic activity.
Biological function summary

The neuraminidase functions as part of the viral replication process. By cleaving sialic acid it prevents viral aggregation at the host cell surface ensuring efficient viral dissemination. This activity complements hemagglutinin another glycoprotein on the viral surface which binds to sialic acid on host cells to initiate infection. Together these proteins orchestrate the infection process marking the structural and functional complexity of the virus.

Pathways

Neuraminidase plays an integral role in the viral lifecycle pathway of the Influenza B virus. It closely interacts with the HA/NA balance where hemagglutinin (HA) binding necessitates subsequent neuraminidase activity to liberate progeny virions. This interaction is critical for maintaining the balance of viral attachment and release during infection influencing the overall efficiency of viral propagation.

The neuraminidase of the Influenza B virus is a significant target in antiviral drug design particularly in relation to influenza infections. Drugs like oseltamivir target neuraminidase to block its activity reducing viral spread and severity of infection. Furthermore the activity of neuraminidase is associated with resistance mutations influencing the effectiveness of antiviral treatment therefore posing significant challenges in combating influenza outbreaks.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Influenza B Virus Neuraminidase
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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