Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27]

6 product images

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  Immunoprecipitation - Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27] (ab313885)

  Influenza B virus Nucleoprotein was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) cells transfected with an Influenza B virus NP gene expression vector containing a myc-His-tag® whole cell lysate with ab313885 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313885 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an Influenza B virus NP gene expression vector containing a myc-His-tag® whole cell lysate
  Lane 2: abab313885 IP in 293T (human embryonic kidney epithelial cell) cells transfected with an Influenza B virus NP gene expression vector containing a myc-His-tag® whole cell lysate
  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313885 in 293T cells transfected with an Influenza B virus NP gene expression vector containing a myc-His-tag® whole cell lysate.
  Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 180 seconds

  All lanes: Immunoprecipitation - Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27] (ab313885) at 1/30 dilution

  All lanes: 293T (human embryonic kidney epithelial cell) cells transfected with an Influenza B virus NP gene expression vector containing a myc-His-tag® whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 180s

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  Western blot - Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27] (ab313885)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  
  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
  In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution
  Exposure time: 26 seconds

  All lanes: Western blot - Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27] (ab313885) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

  Lane 2: 293T cells transfected with an Influenza B virus NP gene expression vector containing a myc-His-tag® whole cell lysate at 5 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 62 kDa, 65 kDa

  Exposure time: 26s

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  Immunocytochemistry/ Immunofluorescence - Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27] (ab313885)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with an Influenza B virus NP gene expression vector containing a myc tag cells labelling Influenza B virus Nucleoprotein with ab313885 at 1/50 (10.16 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing nuclear staining in 293T cells transfected with an Influenza B virus NP gene expression vector containing a myc tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8) Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain at 1/100 (0.38 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

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  Indirect ELISA - Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27] (ab313885)

  Indirect ELISA analysis of abab313885 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution. Antigen: Influenza B virus Nucleoprotein. Antigen concentration: 1000 ng/ml

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27] (ab313885)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Influenza B virus Nucleoprotein with ab313885 at 1/2000 (0.254 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human kidney. The section was incubated with ab313885 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza B virus Nucleoprotein antibody [EPR28205-27] (ab313885)

  Immunohistochemical analysis of paraffin-embedded 293T (human tissue labeling Influenza B virus Nucleoprotein with ab313885 at 1/2000 (0.254 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on (A) 293T transfected with an Influenza B virus NP expression vector and no staining on (B) 293T transfected with empty vector. The section was incubated with ab313885 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins