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AB308414

Anti-Influenza B virus Nucleoprotein antibody [HL1073]

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Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for I-ELISA, ICC/IF, WB and reacts with Recombinant full length protein - Influenza B, Transfected cell line - Influenza B samples.

View Alternative Names

Influenza B Virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Influenza B virus Nucleoprotein using ab308414 at 1/2000 dilution (green).

Western blot - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)
  • WB

Supplier Data

Western blot - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)

Non-transfected (–) and transfected (+) HEK293T whole cell lysate (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1073] (ab308414) diluted at 1/50000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (ab308414) at 1/50000 dilution

Lane 1:

Non-transfected (–) HEK293T whole cell lysate at 30 µg

Lane 2:

Transfected (+) HEK293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Immunocytochemistry/ Immunofluorescence - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)

For immunofluorescence analysis, Influenza A and B Virus infected cells slide were permeabilized for detection of Influenza B virus Nucleoprotein using ab308414 at 1/100 dilution (green).

Indirect ELISA - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 μg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1073] (ab308414) (1 μg/mL). Rabbit IgG antibody (HRP) (1/10000) was used to detect bound primary antibody.

Indirect ELISA - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1073] (ab308414) (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (1/10000) was used to detect bound primary antibody.

Western blot - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)
  • WB

Supplier Data

Western blot - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (AB308414)

Influenza B viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1073] (ab308414) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Influenza B virus Nucleoprotein antibody [HL1073] (ab308414) at 1/1000 dilution

Lane 1:

B/Brisbane/33/08 viral lysate at 1 µg

Lane 2:

B/Florida/02/06 viral lysate at 1 µg

Lane 3:

B/Florida/04/06 viral lysate at 1 µg

Lane 4:

B/Florida/07/04 viral lysate at 1 µg

Lane 5:

B/Malaysia/2506/04 viral lysate at 1 µg

Lane 6:

B/Massachusetts/2/12 viral lysate at 1 µg

Lane 7:

B/Panama/45/90 viral lysate at 1 µg

Lane 8:

B/Wisconsin/1/10 viral lysate at 1 µg

Lane 9:

B/Lee/40 viral lysate at 1 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1073

Isotype

IgG

Carrier free

Yes

Reacts with

Influenza B

Applications

I-ELISA, WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Influenza B": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/2000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Purified native protein - Influenza A": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Influenza B": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Influenza B": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/2000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Influenza B Virus Nucleoprotein often known as NP or flu B NP is an essential structural protein with a molecular weight of approximately 56 kDa. It plays a role in the influenza B virus lifecycle by encapsidating the viral RNA forming the ribonucleoprotein complex important for viral replication and transcription. This protein expresses in the host cell's cytoplasm and nucleus facilitating the export of vRNPs from the nucleus.
Biological function summary

The ribonucleoprotein complex formation involves nucleoproteins assembling with the viral RNA and polymerase subunits securing the genome for replication and transcription. NP interacts with both the polymerase basic 2 (PB2) and polymerase acidic (PA) proteins forming a vital part of the complex. This interaction allows the viral RNA to maintain its structure and function effectively during the virus infection cycle aiding both Influenza A and B viruses.

Pathways

NP participates in the viral replication pathway supporting the influenza virus propagation in infected cells. It associates closely with viral RNA polymerases and is integral to the transcription and replication of the viral RNA genome. Other key proteins involved in these pathways include the polymerase basic 1 (PB1) protein essential for synthesizing viral mRNA and the matrix protein (M1) which aids in vRNA encapsidation and budding.

NP relates closely to influenza B infections contributing to the clinical symptoms of flu B such as fever and respiratory issues. This protein is also a potential target for vaccine and therapeutic development against influenza B. NP shares a connection with the M2 protein another target in antiviral strategies given its role in proton channel activity and viral uncoating. Understanding NP's role can guide the creation of flu B antibodies and influenza A and B antibodies to reduce disease severity or prevent flu infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Influenza B Virus Nucleoprotein
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Product promise

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For full details, please see our Terms & Conditions

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