AB101278

Anti-Influenza Virus NS1A Binding Protein antibody

Name: Anti-Influenza Virus NS1A Binding Protein antibody (ab101278) | Abcam
Brand: Abcam Limited
SKU: AB101278
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal Influenza Virus NS1A Binding Protein antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IVNS1ABP aa 50-100.

View Alternative Names

ARA3, FLARA3, KIAA0850, KLHL39, NS1, NS1BP, HSPC068, IVNS1ABP, Influenza virus NS1A-binding protein, NS1-BP, NS1-binding protein, Aryl hydrocarbon receptor-associated protein 3, Kelch-like protein 39

2 Images

Unknown

Immunoprecipitation - Anti-Influenza Virus NS1A Binding Protein antibody (AB101278)

Detection of Influenza Virus NS1A Binding Protein by Western Blot of Immunprecipitate.
ab101278, at 1 µg/ml, staining Influenza Virus NS1A Binding Protein in HeLa whole cell lysate immunoprecipitated using ab101278 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Influenza Virus NS1A Binding Protein antibody (ab101278)

Predicted band size: 105 kDa,107 kDa,11 kDa,115 kDa,15 kDa,17 kDa,22 kDa,24 kDa,25 kDa,36 kDa,49 kDa,52 kDa,60 kDa,71 kDa,9 kDa,95 kDa

Observed band size: 107 kDa,130 kDa,15 kDa,17 kDa,25 kDa,27 kDa,35 kDa,50 kDa,52 kDa,90 kDa

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Unknown

Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (AB101278)

All lanes:

Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (ab101278) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 71 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human IVNS1ABP aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6Y0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab101278 was affinity purified using an epitope specific to Influenza Virus NS1A Binding Protein immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza Virus NS1A Binding Protein (IVNS1ABP) also known as NS1-BP is a cellular protein that interacts with the NS1 protein of the influenza virus to modulate viral replication. This interaction suppresses host antiviral responses enhancing influenza virus propagation. IVNS1ABP has a molecular weight of approximately 77 kDa. It expresses mainly in the nucleus and cytoplasm of host cells where it engages with host molecules to facilitate viral protein synthesis and assembly.

Biological function summary

IVNS1ABP contributes to the regulation of protein synthesis pathways and facilitates RNA processing by directly interacting with host machinery. It functions within a complex containing several host proteins that assist in transcriptional control and mRNA splicing. These interactions provide the influenza virus with a supportive environment for efficient propagation effectively avoiding the host's immune defenses.

Pathways

IVNS1ABP operates in the RNA processing and splicing mechanisms that are critical for both host and viral gene expression. The protein forms pathways with host RNA polymerase II and other spliceosomal components influencing the maturation of mRNA precursors. These pathways not only support viral replication but also adjust host cell pathways to prioritize viral transcription over normal cellular processes.

IVNS1ABP's modification of host defenses links it directly to influenza pathogenesis and secondary immune suppression. This protein also connects to the nonstructural NS1 protein of the influenza virus an interaction important for evading the host immune response. Understanding IVNS1ABP's role in these pathways helps develop therapeutic strategies for controlling influenza infections targeting its interactions to hinder the viral lifecycle.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. Plays a role in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats (By similarity). Protects cells from cell death induced by actin destabilization (By similarity). Functions as modifier of the AHR/Aryl hydrocarbon receptor pathway increasing the concentration of AHR available to activate transcription (PubMed : 16582008). In addition, functions as a negative regulator of BCR(KLHL20) E3 ubiquitin ligase complex to prevent ubiquitin-mediated proteolysis of PML and DAPK1, two tumor suppressors (PubMed : 25619834). Inhibits pre-mRNA splicing (in vitro) (PubMed : 9696811). May play a role in mRNA nuclear export (PubMed : 30538201).. (Microbial infection) Involved in the alternative splicing of influenza A virus M1 mRNA through interaction with HNRNPK, thereby facilitating the generation of viral M2 protein (PubMed : 23825951, PubMed : 9696811). The BTB and Kelch domains are required for splicing activity (PubMed : 30538201). Promotes export of viral M mRNA and RNP via its interaction with mRNA export factor ALYREF (PubMed : 30538201).

See full target information IVNS1ABP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 16:1401-1414 PubMed38715837

2024

Circ_0072088 promotes breast carcinoma progression via modulating miR-607/RNF2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxi Chen,Yang Hui,Bo Tian

View all publications

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Product promise

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Anti-Influenza Virus NS1A Binding Protein antibody

Immunoprecipitation - Anti-Influenza Virus NS1A Binding Protein antibody (AB101278)

Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (AB101278)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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