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AB127566

Anti-Influenza Virus NS1A Binding Protein antibody

4

(1 Review)

|

(5 Publications)

Rabbit Polyclonal Influenza Virus NS1A Binding Protein antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IVNS1ABP aa 400-600.

View Alternative Names

ARA3, FLARA3, KIAA0850, KLHL39, NS1, NS1BP, HSPC068, IVNS1ABP, Influenza virus NS1A-binding protein, NS1-BP, NS1-binding protein, Aryl hydrocarbon receptor-associated protein 3, Kelch-like protein 39

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza Virus NS1A Binding Protein antibody (AB127566)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza Virus NS1A Binding Protein antibody (AB127566)

ab127566, at 1/500 dilution, staining Influenza Virus NS1A Binding Protein in Human Huh7 xenograft tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza Virus NS1A Binding Protein antibody (AB127566)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza Virus NS1A Binding Protein antibody (AB127566)

ab127566, at 1/500 dilution, staining Influenza Virus NS1A Binding Protein in paraffin-embedded Mouse muscle tissue by Immunohistochemistry.

Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (AB127566)
  • WB

Unknown

Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (AB127566)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (ab127566) at 1/1000 dilution

All lanes:

U-87 MG whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IVNS1ABP aa 400-600. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6Y0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza Virus NS1A Binding Protein (IVNS1ABP) also known as NS1-BP is a cellular protein that interacts with the NS1 protein of the influenza virus to modulate viral replication. This interaction suppresses host antiviral responses enhancing influenza virus propagation. IVNS1ABP has a molecular weight of approximately 77 kDa. It expresses mainly in the nucleus and cytoplasm of host cells where it engages with host molecules to facilitate viral protein synthesis and assembly.
Biological function summary

IVNS1ABP contributes to the regulation of protein synthesis pathways and facilitates RNA processing by directly interacting with host machinery. It functions within a complex containing several host proteins that assist in transcriptional control and mRNA splicing. These interactions provide the influenza virus with a supportive environment for efficient propagation effectively avoiding the host's immune defenses.

Pathways

IVNS1ABP operates in the RNA processing and splicing mechanisms that are critical for both host and viral gene expression. The protein forms pathways with host RNA polymerase II and other spliceosomal components influencing the maturation of mRNA precursors. These pathways not only support viral replication but also adjust host cell pathways to prioritize viral transcription over normal cellular processes.

IVNS1ABP's modification of host defenses links it directly to influenza pathogenesis and secondary immune suppression. This protein also connects to the nonstructural NS1 protein of the influenza virus an interaction important for evading the host immune response. Understanding IVNS1ABP's role in these pathways helps develop therapeutic strategies for controlling influenza infections targeting its interactions to hinder the viral lifecycle.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. Plays a role in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats (By similarity). Protects cells from cell death induced by actin destabilization (By similarity). Functions as modifier of the AHR/Aryl hydrocarbon receptor pathway increasing the concentration of AHR available to activate transcription (PubMed : 16582008). In addition, functions as a negative regulator of BCR(KLHL20) E3 ubiquitin ligase complex to prevent ubiquitin-mediated proteolysis of PML and DAPK1, two tumor suppressors (PubMed : 25619834). Inhibits pre-mRNA splicing (in vitro) (PubMed : 9696811). May play a role in mRNA nuclear export (PubMed : 30538201).. (Microbial infection) Involved in the alternative splicing of influenza A virus M1 mRNA through interaction with HNRNPK, thereby facilitating the generation of viral M2 protein (PubMed : 23825951, PubMed : 9696811). The BTB and Kelch domains are required for splicing activity (PubMed : 30538201). Promotes export of viral M mRNA and RNP via its interaction with mRNA export factor ALYREF (PubMed : 30538201).
See full target information IVNS1ABP
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1614012 PubMed40959429

2025

A mitochondrial ferroptosis-related gene signature predicts prognosis and immune landscape in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hou Wang

Journal of virology 98:e0125823 PubMed38546211

2024

Exploring the therapeutic potential of DV-B-120 as an inhibitor of dengue virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Jung Huang,Tian-Lu Cheng,Yen-Tseng Wang,Chien-Shu Chen,Yu-Ling Leu,Chih-Shiang Chang,Cheng-Han Ho,Shi-Wei Chao,Chia-Tse Li,Chih-Hung Chuang

Cell reports 36:109491 PubMed34348154

2021

Exocyst protein subnetworks integrate Hippo and mTOR signaling to promote virus detection and cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aubhishek Zaman,Xiaofeng Wu,Andrew Lemoff,Sivaramakrishna Yadavalli,Jeon Lee,Chensu Wang,Jonathan Cooper,Elizabeth A McMillan,Charles Yeaman,Hamid Mirzaei,Michael A White,Trever G Bivona

OncoTargets and therapy 14:1589-1604 PubMed33688206

2021

An Apoptotic Gene Signature for the Prognosis of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kunlun Chen,Pengfei Zhu,Yuan Liao,Lei Yan,Ruo Feng,Wenlong Zhai

Oncogene 34:5141-51 PubMed25619834

2015

KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

H Y Chen,J Y Hu,T H Chen,Y C Lin,X Liu,M Y Lin,Y D Lang,Y Yen,R H Chen
View all publications
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Product promise

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