Rabbit Polyclonal Influenza Virus NS1A Binding Protein antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IVNS1ABP aa 400-600.
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Publications
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- OncoTargets and therapy 14:1589-16042021An Apoptotic Gene Signature for the Prognosis of Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKunlun Chen,Pengfei Zhu,Yuan Liao,Lei Yan,Ruo Feng,Wenlong ZhaiPubMed 33688206
- Oncogene 34:5141-512015KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH Y Chen et. al.PubMed 25619834
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IVNS1ABP aa 400-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6Y0
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. Plays a role in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats (By similarity). Protects cells from cell death induced by actin destabilization (By similarity). Functions as modifier of the AHR/Aryl hydrocarbon receptor pathway increasing the concentration of AHR available to activate transcription (PubMed:16582008). In addition, functions as a negative regulator of BCR(KLHL20) E3 ubiquitin ligase complex to prevent ubiquitin-mediated proteolysis of PML and DAPK1, two tumor suppressors (PubMed:25619834). Inhibits pre-mRNA splicing (in vitro) (PubMed:9696811). May play a role in mRNA nuclear export (PubMed:30538201). (Microbial infection) Involved in the alternative splicing of influenza A virus M1 mRNA through interaction with HNRNPK, thereby facilitating the generation of viral M2 protein (PubMed:23825951, PubMed:9696811). The BTB and Kelch domains are required for splicing activity (PubMed:30538201). Promotes export of viral M mRNA and RNP via its interaction with mRNA export factor ALYREF (PubMed:30538201).
Alternative names
ARA3, FLARA3, KIAA0850, KLHL39, NS1, NS1BP, HSPC068, IVNS1ABP, Influenza virus NS1A-binding protein, NS1-BP, NS1-binding protein, Aryl hydrocarbon receptor-associated protein 3, Kelch-like protein 39
Recommended products
Rabbit Polyclonal Influenza Virus NS1A Binding Protein antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IVNS1ABP aa 400-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IVNS1ABP aa 400-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6Y0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Influenza Virus NS1A Binding Protein (IVNS1ABP) also known as NS1-BP is a cellular protein that interacts with the NS1 protein of the influenza virus to modulate viral replication. This interaction suppresses host antiviral responses enhancing influenza virus propagation. IVNS1ABP has a molecular weight of approximately 77 kDa. It expresses mainly in the nucleus and cytoplasm of host cells where it engages with host molecules to facilitate viral protein synthesis and assembly.
- Biological function summary
IVNS1ABP contributes to the regulation of protein synthesis pathways and facilitates RNA processing by directly interacting with host machinery. It functions within a complex containing several host proteins that assist in transcriptional control and mRNA splicing. These interactions provide the influenza virus with a supportive environment for efficient propagation effectively avoiding the host's immune defenses.
- Pathways
IVNS1ABP operates in the RNA processing and splicing mechanisms that are critical for both host and viral gene expression. The protein forms pathways with host RNA polymerase II and other spliceosomal components influencing the maturation of mRNA precursors. These pathways not only support viral replication but also adjust host cell pathways to prioritize viral transcription over normal cellular processes.
- Associated diseases and disorders
IVNS1ABP's modification of host defenses links it directly to influenza pathogenesis and secondary immune suppression. This protein also connects to the nonstructural NS1 protein of the influenza virus an interaction important for evading the host immune response. Understanding IVNS1ABP's role in these pathways helps develop therapeutic strategies for controlling influenza infections targeting its interactions to hinder the viral lifecycle.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza Virus NS1A Binding Protein antibody (ab127566)
ab127566, at 1/500 dilution, staining Influenza Virus NS1A Binding Protein in paraffin-embedded Mouse muscle tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza Virus NS1A Binding Protein antibody (ab127566)
ab127566, at 1/500 dilution, staining Influenza Virus NS1A Binding Protein in Human Huh7 xenograft tissue by Immunohistochemistry.
Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (ab127566)
7.5% SDS PAGE
All lanes: Western blot - Anti-Influenza Virus NS1A Binding Protein antibody (ab127566) at 1/1000 dilution
All lanes: U-87 MG whole cell lysate at 30 µg
Predicted band size: 71 kDa
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